Anticorps Polyclonal de lapin anti-COX4I2

• Phare
• Validé par KD/KO

COX4I2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 11463-1-AP

Synonymes

COX IV 2, COX4, COX4 2, COX4B, COX4I2, COX4L2, COXIV 2

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• Galerie de données de validation
• Proteintech vs. Other Vendors

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  WB analysis using 11463-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1500 incubated at 4 degree celsius over night.

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  WB analysis of human brain using 11463-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11463-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 11463-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11463-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11463-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11463-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11463-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 11463-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment using 11463-1-AP

  IP result of anti-COX4I2 (11463-1-AP for IP and Detection) with HepG2 cell lysate.

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  IP experiment of HepG2 using 11463-1-AP

  IP Result of anti-COX4I2 (IP:11463-1-AP, 3ug; Detection:11463-1-AP 1:1000) with HepG2 cells lysate 8000ug.

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  IHC staining of human ovary tumor using 11463-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11463-1-AP (COX4I2 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 11463-1-AP

  Immunofluorescent analysis of Hela cells, using COX4I2 antibody 11463-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A375, cellules MCF-7, tissu cardiaque humain, tissu cérébral humain, tissu pulmonaire humain
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Informations sur le produit

11463-1-AP cible COX4I2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COX4I2 Protéine recombinante Ag1987
• Nom complet: cytochrome c oxidase subunit IV isoform 2 (lung)
• Masse moléculaire calculée: 171 aa, 20 kDa
• Poids moléculaire observé: 17-18 kDa
• Numéro d’acquisition GenBank: BC057779
• Symbole du gène: COX4I2
• Identification du gène (NCBI): 84701
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

COX4I2, also named as COX4L2 and COXIV-2, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COXIV(cytochrome c oxidase IV) has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. This antibody was generated against full length COX4I2 protein.

Publications

Species	Application	Title
mouse	WB	Cell Rep; Defective Mitochondrial Cardiolipin Remodeling Dampens HIF-1α Expression in Hypoxia.; Authors - Arpita Chowdhury
human	WB	Oncotarget; Nuclear-encoded cytochrome c oxidase subunit 4 regulates BMI1 expression and determines proliferative capacity of high-grade gliomas.; Authors - Claudia R Oliva ; KD Validated
mouse	IF	Sci Signal; Acute O2 sensing through HIF2α-dependent expression of atypical cytochrome oxidase subunits in arterial chemoreceptors.; Authors - Alejandro Moreno-Domínguez ; KO Validated
human	WB	Free Radic Biol Med; Cytochrome c oxidase mediates labile iron level and radioresistance in glioblastoma.; Authors - Md Yousuf Ali
human	WB	Bioconjug Chem; GnRH-Gemcitabine Conjugates for the Treatment of Androgen-Independent Prostate Cancer: Pharmacokinetic Enhancements Combined with Targeted Drug Delivery.; Authors - Theodoros Karampelas
human	WB	Free Radic Biol Med; Differentiation of SH-SY5Y cells to a neuronal phenotype changes cellular bioenergetics and the response to oxidative stress.; Authors - Schneider Lonnie L