Anticorps Polyclonal de lapin anti-COX7C

• Phare
• Validé par KD/KO

COX7C Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 11411-2-AP

Synonymes

Cytochrome c oxidase subunit 7C, mitochondrial

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Galerie de données de validation

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  WB analysis of mouse ovary using 11411-2-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11411-2-AP (COX7C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 11411-2-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11411-2-AP (COX7C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 11411-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11411-2-AP (COX7C antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of MCF-7 using 11411-2-AP

  Immunofluorescent analysis of MCF-7 cells, using COX7C antibody 11411-2-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: tissu ovarien de souris, tissu de muscle squelettique humain
• Résultats positifs en IHC: tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 2 publications below

Informations sur le produit

11411-2-AP cible COX7C dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COX7C Protéine recombinante Ag1989
• Nom complet: cytochrome c oxidase subunit VIIc
• Masse moléculaire calculée: 63 aa, 7 kDa
• Poids moléculaire observé: 15~28 kDa
• Numéro d’acquisition GenBank: BC007498
• Symbole du gène: COX7C
• Identification du gène (NCBI): 1350
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. COX7C (Cytochrome c oxidase subunit 7C, mitochondrial) is one of the subunits, catalyzing the elctron transfer from reduced cytochrome c to molecule oxygen. This protein belongs to the cytochrome c oxidase VIIc family. The calculated molecular weight of COX7C is 7 kDa, but due to the presence of the complex, a complex form of 28 kDa may also be observed.

Publications

Species	Application	Title
human	WB	Cell Death Dis; O-GlcNAcylation on Rab3A attenuates its effects on mitochondrial oxidative phosphorylation and metastasis in hepatocellular carcinoma.; Authors - Weicheng Wu
human	WB	J Biol Chem; PMID: 20870728; Authors - Oliva Claudia R CR
human	WB	Cancer Sci; Urea transport B gene induces melanoma B16 cell death via activation of p53 and mitochondrial apoptosis.; Authors - Lianqin Liu
human	IF	J Neuropathol Exp Neurol; Altered Mitochondria, Protein Synthesis Machinery, and Purine Metabolism Are Molecular Contributors to the Pathogenesis of Creutzfeldt-Jakob Disease.; Authors - Belén Ansoleaga
mouse	WB,IF	Front Pharmacol; Effect of Dl-3-n-butylphthalide on mitochondrial Cox7c in models of cerebral ischemia/reperfusion injury; Authors - Jingjing Jia ; KD Validated
mouse	WB	Nat Commun; ZHX2 emerges as a negative regulator of mitochondrial oxidative phosphorylation during acute liver injury; Authors - Yankun Zhang

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