Anticorps Polyclonal de lapin anti-COX8A

COX8A Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(5)

Avis (1)

N° de cat : 15368-1-AP

Synonymes

COX8, COX8 2, COX8L, Cytochrome c oxidase polypeptide VIII-liver/heart, Cytochrome c oxidase subunit 8-2

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Galerie de données de validation

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  WB analysis of mouse skeletal muscle using 15368-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15368-1-AP (COX8A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 15368-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15368-1-AP (COX8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat heart using 15368-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 15368-1-AP (COX8A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 15368-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 15368-1-AP (COX8A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 15368-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15368-1-AP (COX8A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 15368-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15368-1-AP (COX8A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 15368-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15368-1-AP (COX8A antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, tissu de muscle squelettique humain
• Résultats positifs en IHC: tissu cardiaque de souris, tissu cardiaque de rat, tissu de cirrhose hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

15368-1-AP cible COX8A dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COX8A Protéine recombinante Ag2885
• Nom complet: cytochrome c oxidase subunit 8A (ubiquitous)
• Masse moléculaire calculée: 8 kDa
• Poids moléculaire observé: 8 kDa
• Numéro d’acquisition GenBank: BC063025
• Symbole du gène: COX8A
• Identification du gène (NCBI): 1351
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

COX8A(Cytochrome c oxidase subunit 8A) is also named as COX8, COX8L and belongs to the cytochrome c oxidase VIII family. This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. The gene encodes a full length protein of 8 kDa with a transit peptide of 25 amino acid.

Publications

Species	Application	Title
human	WB	Cell Death Dis; O-GlcNAcylation on Rab3A attenuates its effects on mitochondrial oxidative phosphorylation and metastasis in hepatocellular carcinoma.; Authors - Weicheng Wu
human	IHC	J Proteomics; MALDI-MS tissue imaging identification of biliverdin reductase B overexpression in prostate cancer.; Authors - Pallua Johannes Dominikus JD
mouse	WB	Nat Commun; A FRET-based respirasome assembly screen identifies spleen tyrosine kinase as a target to improve muscle mitochondrial respiration and exercise performance in mice; Authors - Ami Kobayashi
rat	WB	Basic Res Cardiol; Comparison of the stage-dependent mitochondrial changes in response to pressure overload between the diseased right and left ventricle in the rat; Authors - Ling Li
mouse	WB,IF	J Adv Res; Lactational high weight loss impairs follicular development by causing mitochondrial dysfunction of ovarian cells in sows and mitigated by butyrate supplement; Authors - Kexiong Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Svitlana (Verified Customer) (03-01-2019)

HEK293t transfected with Cox8-eGFP, 48 hours post-transfection cells collected for mitochondria isolation. 10 ug mitochondrial lysate (in RIPA buffer) was loaded per line.Incubation with primary anti-Cox8 antibody overnight at 4C.Image: 1st line protein ladder, 2nd line no-transfection sample, 3rd line Cox8-eGFP transfected sample.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293t

COX8A Antibody Western Blot, validation (1:1000 dilution) in HEK293t (Cat no:15368-1-AP)

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