Anticorps Monoclonal anti-COXIV

COXIV Monoclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications(2)

CloneNo.

3C7D2

N° de cat : CL488-60251

Synonymes

COX4I1, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, COX4, COX IV-1, 3C7D2

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Galerie de données de validation

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  IF Staining of HepG2 using CL488-60251

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 COXIV antibody (CL488-60251, Clone: 3C7D2 ) at dilution of 1:200, CL594-phalloidin (red).

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  FC experiment of HeLa using CL488-60251

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human COXIV (CL488-60251, Clone:3C7D2) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

CL488-60251 cible COXIV dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: COXIV Protéine recombinante Ag20551
• Nom complet: cytochrome c oxidase subunit IV isoform 1
• Masse moléculaire calculée: 19.6 kDa
• Poids moléculaire observé: 17-18 kDa
• Numéro d’acquisition GenBank: BC021236
• Symbole du gène: COX IV
• Identification du gène (NCBI): 1327
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody is specific to COX4I1 and do not cross reacts with COX4I2.

Publications

Species	Application	Title
mouse	FC	Nat Immunol; Dynamic mitochondrial transcription and translation in B cells control germinal center entry and lymphomagenesis; Authors - Yavuz F Yazicioglu
mouse	IF	Life Sci; Short-term but not long-term high fat diet feeding protects against pressure overload-induced heart failure through activation of mitophagy.; Authors - Yanzhen Tan

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