Anticorps Polyclonal de lapin anti-Ceruloplasmin

Ceruloplasmin Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 21131-1-AP

Synonymes

Ceruloplasmin, ceruloplasmin (ferroxidase), CP, CP 2, Ferroxidase

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Galerie de données de validation

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  WB analysis of human plasma using 21131-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 21131-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21131-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human hepatocirrhosis using 21131-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 21131-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human tonsillitis using 21131-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21131-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu sanguin humain, tissu cérébral de souris, tissu plasmatique humain
• Résultats positifs en IHC: tissu de cirrhose hépatique humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

21131-1-AP cible Ceruloplasmin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Ceruloplasmin Protéine recombinante Ag15535
• Nom complet: ceruloplasmin (ferroxidase)
• Masse moléculaire calculée: 1065 aa, 122 kDa
• Poids moléculaire observé: 130-140 kDa, 85 kDa
• Numéro d’acquisition GenBank: BC146663
• Symbole du gène: CP
• Identification du gène (NCBI): 1356
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ceruloplasmin (CP) is a serum glycoprotein synthesized by hepatocytes and functions as the major transport protein of copper in blood where it acts as ferrodoxidase and is required for iron transport of transferrin. The intact ceruloplasmin migrated around 130-140 kDa and some proteolytic cleaved fragments could also be observed.

Publications

Species	Application	Title
human	WB,IHC	Cells; Phaseolus vulgaris Erythroagglutinin (PHA-E)-Positive Ceruloplasmin Acts as a Potential Biomarker in Pancreatic Cancer Diagnosis; Authors - Shanshan Sha
human	WB	J Clin Pharmacol; Expression of ceruloplasmin in the peripheral blood of patients with drug-resistant epilepsy; Authors - Hai-Yan Chen
mouse	WB,IF	Cell Death Dis; The divergent effects of astrocyte ceruloplasmin on learning and memory function in young and old mice; Authors - Zhong-Da Li
mouse	WB	iScience; Hepatic depletion of nucleolar protein mDEF causes excessive mitochondrial copper accumulation associated with p53 and NRF1 activation; Authors - Jinsong Wei
mouse	WB	Mol Neurobiol; Gemfibrozil Alleviates Cognitive Impairment by Inhibiting Ferroptosis of Astrocytes via Restoring the Iron Metabolism and Promoting Antioxidant Capacity in Type 2 Diabetes; Authors - Nan Wang