Anticorps Polyclonal de lapin anti-Ceruloplasmin

• Phare
• Validé par KD/KO

Ceruloplasmin Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(11)

N° de cat : 21131-1-AP

Synonymes

CP, CERU, Cuproxidase ceruloplasmin, EC:1.11.1.27, EC:1.11.1.9

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Galerie de données de validation

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  WB analysis of human plasma using 21131-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 21131-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21131-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 21131-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 21131-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 21131-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 21131-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 21131-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu sanguin humain, tissu cérébral de souris, tissu plasmatique humain
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cirrhose hépatique humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

21131-1-AP cible Ceruloplasmin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Ceruloplasmin Protéine recombinante Ag15535
• Nom complet: ceruloplasmin (ferroxidase)
• Masse moléculaire calculée: 1065 aa, 122 kDa
• Poids moléculaire observé: 130-140 kDa, 85 kDa
• Numéro d’acquisition GenBank: BC146663
• Symbole du gène: Ceruloplasmin
• Identification du gène (NCBI): 1356
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ceruloplasmin (CP) is a serum glycoprotein synthesized by hepatocytes and functions as the major transport protein of copper in blood where it acts as ferrodoxidase and is required for iron transport of transferrin. The intact ceruloplasmin migrated around 130-140 kDa and some proteolytic cleaved fragments could also be observed.

Publications

Species	Application	Title
mouse,human	WB,IHC	Int J Biol Sci; Novel LncRNA LINC02936 Suppresses Ferroptosis and Promotes Tumor Progression by Interacting with SIX1/CP Axis in Endometrial Cancer; Authors - Zihui Zhang ; KD Validated
human	WB	Carcinogenesis; Identification of HCAR1 as a ferroptosis-related biomarker of gastric cancer based on a novel ferroptosis-related prognostic model and in vitro experiments; Authors - Hongjiao Zhang
chicken	IHC,IF	J Agric Food Chem; Drp1 Aggravates Copper Nanoparticle-Induced ER-Phagy by Disturbing Mitochondria-Associated Membranes in Chicken Hepatocytes; Authors - Quanwei Li
mouse	WB	J Neuroinflammation; Aberrant copper metabolism and hepatic inflammation cause neurological manifestations in a mouse model of Wilson's disease; Authors - Jianjian Dong
mouse	WB	Toxicology; Benzyl butyl phthalate promotes ferroptosis in Sertoli cells via disrupting ceruloplasmin-mediated iron balance; Authors - Yu Dong
human	WB,IHC	Cells; Phaseolus vulgaris Erythroagglutinin (PHA-E)-Positive Ceruloplasmin Acts as a Potential Biomarker in Pancreatic Cancer Diagnosis; Authors - Shanshan Sha

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