Anticorps Polyclonal de lapin anti-Carboxypeptidase A1

Carboxypeptidase A1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 15836-1-AP

Synonymes

Carboxypeptidase A1, CPA, CPA1

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Galerie de données de validation

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  WB analysis of mouse pancreas using 15836-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 15836-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 15836-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 15836-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 15836-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 15836-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 15836-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 15836-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 15836-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 15836-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 15836-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15836-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu pancréatique de souris
• Résultats positifs en IHC: tissu placentaire humain, tissu hépatique humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below
• IF: See 4 publications below

Informations sur le produit

15836-1-AP cible Carboxypeptidase A1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Carboxypeptidase A1 Protéine recombinante Ag8586
• Nom complet: carboxypeptidase A1 (pancreatic)
• Masse moléculaire calculée: 419 aa, 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC005279
• Symbole du gène: CPA1
• Identification du gène (NCBI): 1357
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CPA1(Carboxypeptidase A1) is also named as CPA and belongs to the the peptidase M14 family. CPA1 is one of the genes whose expression in the pancreas and the protein which it encodes is synthesized as an inactive precursor, proCPA1, which is processed to the active enzyme by the proteolytic removal of the 95-amino acid N-terminal prodomain. The mature CPA1 is not secreted and is trapped and degraded intracellularly(PMID:8639628). This antibody is speicific to CPA1.

Publications

Species	Application	Title
mouse	IHC	Cell Rep; αE-Catenin Is a Positive Regulator of Pancreatic Islet Cell Lineage Differentiation.; Authors - Antonio J Jimenez-Caliani
mouse	IF	Development; Focal adhesion kinase-mediated signaling controls the onset of pancreatic cell differentiation; Authors - Uylissa A Rodriguez
rat	IF	J Nutr Biochem; Intermittent protein restriction improves glucose homeostasis in Zucker diabetic fatty rats and single-cell sequencing reveals distinct changes in β cells; Authors - Chenchen Li
mouse	IF	Pancreatology; Smad4 deficiency substitutes Cdkn2b but not Cdkn2a downregulation in pancreatic cancer following induction of genetic events in adult mice.; Authors - Xintong Jia
	IF	Dis Model Mech; A novel pancreatic cancer model originated from transformation of acinar cells in adult tree shrew, a primate-like animal.; Authors - Qiu Tu