Anticorps Polyclonal de lapin anti-CPE

• Phare
• Validé par KD/KO

CPE Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(13)

N° de cat : 13710-1-AP

Synonymes

Carboxypeptidase E, Carboxypeptidase H, CPH, EC:3.4.17.10, Prohormone-processing carboxypeptidase

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Galerie de données de validation

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  WB analysis of mouse brain using 13710-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13710-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 13710-1-AP

  IP result of anti-Carboxypeptidase E (IP:13710-1-AP, 3ug; Detection:13710-1-AP 1:500) with mouse brain tissue lysate 3500ug.

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  IHC staining of mouse brain using 13710-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13710-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 13710-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pituitary using 13710-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pituitary using 13710-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 13710-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Carboxypeptidase E antibody (13710-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 13710-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Carboxypeptidase E antibody (13710-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris,
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral de rat, tissu hypophysaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 4 publications below

Informations sur le produit

13710-1-AP cible CPE dans les applications de WB, IHC, IF/ICC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CPE Protéine recombinante Ag4647
• Nom complet: carboxypeptidase E
• Masse moléculaire calculée: 476 aa, 53 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC033866
• Symbole du gène: Carboxypeptidase E
• Identification du gène (NCBI): 1363
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CPE(Carboxypeptidase E) is also named as CPH.It belongs to the peptidase M14 family and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin.The deduced 476-amino acid human protein contains an N-terminal signal peptide, followed by a 'pro' sequence, a polyarginine stretch, and 2 potential N-glycosylation sites.

Publications

Species	Application	Title
rat	WB	Cell Metab; Reductive TCA cycle metabolism fuels glutamine- and glucose-stimulated insulin secretion.; Authors - Guo-Fang Zhang
mouse	WB	Diabetes; Translational Factor eIF4G1 Regulates Glucose Homeostasis and Pancreatic β-Cell Function.; Authors - Seokwon Jo
		Am J Physiol Endocrinol Metab; Loss of the Golgi-localized v-ATPase subunit does not alter insulin granule formation or pancreatic islet β-cell function; Authors - Cierra K Boyer
mouse,human	IHC	Brain Pathol; Secretory sorting receptors carboxypeptidase E and secretogranin III in amyloid β-associated neural degeneration in Alzheimer's disease.; Authors - Virginia Plá
mouse	WB	Neuropharmacology; Esketamine mitigates endotoxin-induced hippocampal injury by regulating calcium transient and synaptic plasticity via the NF-α1/CREB pathway; Authors - Mu Xu
human	WB,IHC	Front Genet; Development and Validation of a Novel Gene Signature for Predicting the Prognosis by Identifying m5C Modification Subtypes of Cervical Cancer.; Authors - Jing Yu ; KD Validated

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