Anticorps Polyclonal de lapin anti-CPEB1

• Phare
• Validé par KD/KO

CPEB1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 13274-1-AP

Synonymes

CEBP, CPE binding protein 1, CPE BP1, CPEB, CPEB1, h CEBP, hCPEB 1

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Galerie de données de validation

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  WB analysis using 13274-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13274-1-AP (CPEB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 13274-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13274-1-AP (CPEB1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 13274-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13274-1-AP (CPEB1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 13274-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13274-1-AP (CPEB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13274-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13274-1-AP (CPEB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13274-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13274-1-AP (CPEB1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 13274-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13274-1-AP (CPEB1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 13274-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13274-1-AP (CPEB1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 13274-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13274-1-AP (CPEB1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 13274-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CPEB1 antibody (13274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du côlon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• IP: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

13274-1-AP cible CPEB1 dans les applications de WB, RIP, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CPEB1 Protéine recombinante Ag3994
• Nom complet: cytoplasmic polyadenylation element binding protein 1
• Masse moléculaire calculée: 486 aa, 54 kDa
• Poids moléculaire observé: 60-70kDa
• Numéro d’acquisition GenBank: BC035348
• Symbole du gène: CPEB1
• Identification du gène (NCBI): 64506
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CPEB1, also named as CEBP, CPEB and CPE-BP1, contains two RRM (RNA recognition motif) domains and belongs to the RRM CPEB family. It is a sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. CPEB1 regulated translation is a key process involved in INS signaling. This is a rabbit polyclonal antibody raised against residues near the C terminus of human CPEB1.

Publications

Species	Application	Title
human,mouse	WB	Nature; Autism-like phenotype and risk gene mRNA deadenylation by CPEB4 mis-splicing.; Authors - Alberto Parras
human	WB,IF,IP	Nature; CPEB1 coordinates alternative 3'-UTR formation with translational regulation.; Authors - Felice-Alessio Bava ; KD Validated
mouse	WB	Nat Cell Biol; Ultrasensitive Ribo-seq reveals translational landscapes during mammalian oocyte-to-embryo transition and pre-implantation development.; Authors - Zhuqing Xiong
human,mouse,rat	WB,IHC,IP	Gastroenterology; Sequential Functions of CPEB1 and CPEB4 Regulate Pathologic Expression of Vascular Endothelial Growth Factor and Angiogenesis in Chronic Liver Disease.; Authors - Vittorio Calderone ; KD Validated
mouse	WB	Adv Sci (Weinh); Selective Translation of Maternal mRNA by eIF4E1B Controls Oocyte to Embryo Transition; Authors - Jing Guo
human	WB	Nat Commun; Translational reprogramming in tumour cells can generate oncoselectivity in viral therapies.; Authors - Eneko Villanueva

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