Anticorps Polyclonal de lapin anti-Carboxypeptidase M

Carboxypeptidase M Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 16440-1-AP

Synonymes

Carboxypeptidase M, CPM

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Galerie de données de validation

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  WB analysis of HEK-293 using 16440-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16440-1-AP (Carboxypeptidase M antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16440-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16440-1-AP (Carboxypeptidase M antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16440-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16440-1-AP (Carboxypeptidase M antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

16440-1-AP cible Carboxypeptidase M dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Carboxypeptidase M Protéine recombinante Ag9761
• Nom complet: carboxypeptidase M
• Masse moléculaire calculée: 443 aa, 51 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC022276
• Symbole du gène: Carboxypeptidase M
• Identification du gène (NCBI): 1368
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CPM(Carboxypeptidase M), a plasma membrane-bound enzyme, is present in many human organs and differs from other carboxypeptidases that cleave basic C-terminal amino acids(PMID:2394713). CPM is structurally, catalytically and immunologically distinct from other carboxypeptidases, and expressed on cell membranes in a glycosyl-phosphatidylinositol-anchored form and it can be a novel marker and cellular player in lipid uptake and/or metabolism of MAs by promoting foam cell formation (PMID:22157720). This full length protein has a signal peptide with 17 amino acid, a propeptide with 20 amino acid and five glycosylation sites.

Publications

Species	Application	Title
human	WB	Sci Adv; FAM50A drives breast cancer brain metastasis through interaction with C9ORF78 to enhance ʟ-asparagine production; Authors - Weilong Chen

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