Anticorps Polyclonal de lapin anti-CPNE4

CPNE4 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 16075-1-AP

Synonymes

Copine 4, Copine 8, copine IV, COPN4, CPN4, CPNE4

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Galerie de données de validation

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  WB analysis using 16075-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16075-1-AP (CPNE4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 16075-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CPNE4 antibody (16075-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

16075-1-AP cible CPNE4 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CPNE4 Protéine recombinante Ag9037
• Nom complet: copine IV
• Masse moléculaire calculée: 557 aa, 62 kDa
• Poids moléculaire observé: 62 kDa
• Numéro d’acquisition GenBank: BC014396
• Symbole du gène: CPNE4
• Identification du gène (NCBI): 131034
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Copines are a family of evolutionarily conserved calcium-dependent phospholipid-binding proteins (PMID: 9430674). They contain two Ca(2+)- and phospholipid-binding domains known as C2 domains. Copines are potentially involved in regulating membrane trafficking and in protein-protein interactions. CPNE4 (Copine 4) belongs to the copine family. This antibody is specific to CPNE4.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Cajsa (Verified Customer) (09-17-2024)

Great antibody. Image of enteric neuronal nuclei (green, Baf53 reporter) and Cpna4 (red).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Myenteric plexus of adult mouse intestine

CPNE4 Antibody Immunofluorescence validation (1:300 dilution) in Myenteric plexus of adult mouse intestine (Cat no:16075-1-AP)

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