Anticorps Polyclonal de lapin anti-CPOX

• Phare
• Validé par KD/KO

CPOX Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 12211-1-AP

Synonymes

Coprogen oxidase, Coproporphyrinogen oxidase, Coproporphyrinogenase, COX, CPO, CPOX, CPX, HCP

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Galerie de données de validation

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  WB analysis using 12211-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12211-1-AP (CPOX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12211-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12211-1-AP (CPOX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12211-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12211-1-AP (CPOX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12211-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12211-1-AP (CPOX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 12211-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12211-1-AP (CPOX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12211-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12211-1-AP (CPOX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12211-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12211-1-AP (CPOX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 12211-1-AP

  IP Result of anti-CPOX (IP:12211-1-AP, 4ug; Detection:12211-1-AP 1:500) with HepG2 cells lysate 2400ug.

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  IHC staining of human liver cancer using 12211-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12211-1-AP (CPOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12211-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12211-1-AP (CPOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 12211-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12211-1-AP (CPOX antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 12211-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12211-1-AP (CPOX antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver cancer using 12211-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12211-1-AP (CPOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12211-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12211-1-AP (CPOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules Jurkat, cellules K-562, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

12211-1-AP cible CPOX dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CPOX Protéine recombinante Ag2858
• Nom complet: coproporphyrinogen oxidase
• Masse moléculaire calculée: 454 aa, 50 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC023551
• Symbole du gène: CPOX
• Identification du gène (NCBI): 1371
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CPOX(Coproporphyrinogen-III oxidase, mitochondrial) is also named as CPO and CPX. Human CPO is a 76 kDa protein that is active as a homodimer in the mitochondrial intermembrane pace(PMID:16159891).Mutations in human CPO gene predict the clinical outcome of the disease, with either hepatic hereditary coproporphyria or hematological manifestations of erythropoietic harderoporphyria.CPOX has a transit peptide of 110 aa and the chain contains 344 aa.

Publications

Species	Application	Title
human	WB, IF	J Cell Biol; Parkin-independent mitophagy via Drp1-mediated outer membrane severing and inner membrane ubiquitination.; Authors - Yumiko Oshima
human	IHC	Neuro Oncol; Enhanced expression of coproporphyrinogen oxidase in malignant brain tumors: CPOX expression and 5-ALA-induced fluorescence.; Authors - Takahashi Kenkichi K
human,mouse	WB	Biochem Pharmacol; Cystathionine-γ-lyase (CSE) deficiency increases erythropoiesis and promotes mitochondrial electron transport via the upregulation of coproporphyrinogen III oxidase and consequent stimulation of heme biosynthesis.; Authors - Katalin Módis ; KD Validated
human	IHC	Front Med (Lausanne); Heme Biosynthesis Factors and 5-ALA Induced Fluorescence: Analysis of mRNA and Protein Expression in Fluorescing and Non-fluorescing Gliomas.; Authors - Mario Mischkulnig
human	IHC	Photodiagnosis Photodyn Ther; Expression of coproporphyrinogen oxidase is associated with detection of upper gastrointestinal carcinomas by 5-aminolevulinic acid-mediated photodynamic diagnosis.; Authors - Kumi Ogihara
human	IHC	Photodiagnosis Photodyn Ther; Protoporphyrinogen oxidase is involved in the fluorescence intensity of 5-aminolevulinic acid-mediated laser-based photodynamic endoscopic diagnosis for early gastric cancer.; Authors - Hiroki Kurumi