Anticorps Polyclonal de lapin anti-CPSF3

• Phare
• Validé par KD/KO

CPSF3 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 11609-1-AP

Synonymes

CPSF, CPSF 73, CPSF 73 kDa subunit, CPSF3, CPSF73, YSH1

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Galerie de données de validation

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  WB analysis of HEK-293 using 11609-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11609-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human skin using 11609-1-AP

  human skin tissue were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11609-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11609-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 11609-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:700.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, tissu cérébral humain, tissu cutané humain
• Résultats positifs en IP: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below

Informations sur le produit

11609-1-AP cible CPSF3 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CPSF3 Protéine recombinante Ag2173
• Nom complet: cleavage and polyadenylation specific factor 3, 73kDa
• Masse moléculaire calculée: 684 aa, 77 kDa
• Poids moléculaire observé: 77 kDa
• Numéro d’acquisition GenBank: BC020211
• Symbole du gène: CPSF3
• Identification du gène (NCBI): 51692
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	J Exp Clin Cancer Res; LncRNA CASC9 interacts with CPSF3 to regulate TGF-β signaling in colorectal cancer.; Authors - Kaili Luo ; KD Validated
human	WB	Cancers (Basel); CPSF3 Promotes Pre-mRNA Splicing and Prevents CircRNA Cyclization in Hepatocellular Carcinoma; Authors - Ying Huang
human	WB	Cell Rep; Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome.; Authors - J J David Ho
pig	WB	Cell Mol Life Sci; Single-cell multi-omics profiling reveals key regulatory mechanisms that poise germinal vesicle oocytes for maturation in pigs; Authors - Xiaolong Yuan
human	WB	Cell Chem Biol; Anticancer benzoxaboroles block pre-mRNA processing by directly inhibiting CPSF3; Authors - Ye Tao