Anticorps Monoclonal anti-CPT1A

• Phare
• Validé par KD/KO

CPT1A Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(39)

CloneNo.

5D8G9

Avis (1)

N° de cat : 66039-1-Ig

Synonymes

5D8G9, Carnitine O-palmitoyltransferase 1, liver isoform, Carnitine O-palmitoyltransferase I, liver isoform, CPT I, CPT1

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Galerie de données de validation

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  WB analysis of A549 using 66039-1-Ig

  WB result of CPT1A antibody (66039-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPT1A transfected A549 cells.

• 

  IP experiment of MCF-7 using 66039-1-Ig

  IP result of anti-CPT1A (IP:66039-1-Ig, 4ug; Detection:66039-1-Ig 1:500) with MCF-7 cells lysate 1200ug.

• 

  IHC staining of human breast cancer using 66039-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66039-1-Ig (CPT1A antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66039-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66039-1-Ig (CPT1A antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66039-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66039-1-Ig(CPT1A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 66039-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66039-1-Ig(CPT1A antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 66039-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CPT1A antibody (66039-1-Ig, Clone: 5D8G9 ) at dilution of 1:1800 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

• 

  FC experiment of HeLa using 66039-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CPT1A Monoclonal antibody (66039-1-Ig, Clone:5D8G9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A549,
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:900-1:3600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 33 publications below
• IHC: See 6 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

66039-1-Ig cible CPT1A dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CPT1A Protéine recombinante Ag7745
• Nom complet: carnitine palmitoyltransferase 1A (liver)
• Masse moléculaire calculée: 88 kDa
• Poids moléculaire observé: 86 kDa
• Numéro d’acquisition GenBank: BC000185
• Symbole du gène: CPT1A
• Identification du gène (NCBI): 1374
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CPT1A, also named as CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active forms of related liver-type carnitine palmitoyltransferase I. (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.

Publications

Species	Application	Title
	WB	Cancer Cell; Stromal lipid species dictate melanoma metastasis and tropism; Authors - Shilpa Gurung
human	IHC,CoIP	Acta Pharmacol Sin; STING inhibits the progression of esophageal squamous cell carcinoma by suppressing CPT1A-mediated fatty acid β-oxidation; Authors - Lei Zhang
mouse	WB	Diabetes; CEPT1-Mediated Phospholipogenesis Regulates Endothelial Cell Function and Ischemia-Induced Angiogenesis Through PPARα.; Authors - Mohamed A Zayed ; KD Validated
mouse	WB	Phytother Res; Angelica sinensis polysaccharide ameliorates nonalcoholic fatty liver disease via restoring estrogen-related receptor α expression in liver; Authors - Li Luo
mouse	IF	Commun Biol; Cpt1a Drives primed-to-naïve pluripotency transition through lipid remodeling; Authors - Zhaoyi Ma
rat,human	WB	Front Pharmacol; Bushen Huoxue formula attenuates lipid accumulation evoking excessive autophagy in premature ovarian insufficiency rats and palmitic acid-challenged KGN cells by modulating lipid metabolism; Authors - Tian Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mohammad (Verified Customer) (04-29-2019)

working well, Without any non-specific band

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse hippocampus

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