Anticorps Monoclonal anti-CPT1A

• Phare
• Validé par KD/KO

CPT1A Monoclonal Antibody for FC, IHC, IP, WB,ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

18

CloneNo.

5D8G9

Avis (1)

N° de cat : 66039-1-Ig

Synonymes

CPT I, CPT1, CPT1 L, CPT1A, CPTI L, L CPT1

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Galerie de données de validation

• 

  WB analysis of A549 using 66039-1-Ig

  WB result of CPT1A antibody (66039-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPT1A transfected A549 cells.

• 

  IP experiment of MCF-7 using 66039-1-Ig

  IP Result of anti-CPT1A (IP:66039-1-Ig, 4ug; Detection:66039-1-Ig 1:500) with MCF-7 cells lysate 1200ug.

• 

  IHC staining of human breast cancer using 66039-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66039-1-Ig (CPT1A antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66039-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66039-1-Ig (CPT1A antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66039-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66039-1-Ig(CPT1A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 66039-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66039-1-Ig(CPT1A antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HeLa using 66039-1-Ig

  1X10^6 HeLa cells were stained with 0.2ug CPT1A antibody (66039-1-Ig, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Applications testées

• Résultats positifs en WB: cellules A549,
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

66039-1-Ig cible CPT1A dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CPT1A Protéine recombinante Ag7745
• Nom complet: carnitine palmitoyltransferase 1A (liver)
• Masse moléculaire calculée: 88 kDa
• Poids moléculaire observé: 86 kDa
• Numéro d’acquisition GenBank: BC000185
• Symbole du gène: CPT1A
• Identification du gène (NCBI): 1374
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CPT1A, also named as CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active forms of related liver-type carnitine palmitoyltransferase I. (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.

Publications

Species	Application	Title
mouse	WB	Diabetes; CEPT1-Mediated Phospholipogenesis Regulates Endothelial Cell Function and Ischemia-Induced Angiogenesis Through PPARα.; Authors - Mohamed A Zayed ; KD Validated
mouse	WB	Phytother Res; Angelica sinensis polysaccharide ameliorates nonalcoholic fatty liver disease via restoring estrogen-related receptor α expression in liver; Authors - Li Luo
human	WB	Cancer Metab; MicroRNA-377-3p inhibits hepatocellular carcinoma growth and metastasis through negative regulation of CPT1C-mediated fatty acid oxidation.; Authors - Ting Zhang
human	WB,IHC	Front Oncol; Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma.; Authors - Jinghui Lu
mouse	WB	Food Funct; PMID: 32613952; Authors - Shuli Man
mouse	WB,IHC	Food Funct; Hawk tea prevents high-fat diet-induced obesity in mice by activating the AMPK/ACC/SREBP1c signaling pathways and regulating the gut microbiota.; Authors - Wei Tao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mohammad (Verified Customer) (04-29-2019)

working well, Without any non-specific band

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse hippocampus