Anticorps Polyclonal de lapin anti-CPT1B-specific

• Phare
• Validé par KD/KO

CPT1B-specific Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(81)

Avis (2)

N° de cat : 22170-1-AP

Synonymes

CPT1B, Carnitine O palmitoyltransferase 1, muscle isoform, Carnitine O-palmitoyltransferase 1, muscle isoform, Carnitine O-palmitoyltransferase I, muscle isoform, Carnitine palmitoyltransferase I-like protein

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Galerie de données de validation

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  WB analysis using 22170-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22170-1-AP (CPT1B-specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 22170-1-AP

  Rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22170-1-AP (CPT1B-specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 22170-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22170-1-AP (CPT1B-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 22170-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 22170-1-AP (CPT1B-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 22170-1-AP

  IP result of anti-CPT1B-specific (IP:22170-1-AP, 4ug; Detection:22170-1-AP 1:600) with mouse heart tissue lysate 3600 ug.

• 

  IP experiment of mouse skeletal muscle using 22170-1-AP

  IP result of anti-CPT1B-specific (IP:22170-1-AP, 4ug; Detection:22170-1-AP 1:600) with mouse skeletal muscle tissue lysate 2200ug.

• 

  IHC staining of mouse heart using 22170-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22170-1-AP (CPT1B-specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 22170-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22170-1-AP (CPT1B-specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 22170-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using CPT1B-specific antibody (22170-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 22170-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using CPT1B-specific antibody (22170-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque de rat, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu cardiaque de souris, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu cardiaque de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cardiaque de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 75 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

22170-1-AP cible CPT1B-specific dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, porc, souris, Brebis
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CPT1B-specific Protéine recombinante Ag17840
• Nom complet: carnitine palmitoyltransferase 1B (muscle)
• Masse moléculaire calculée: 567 aa, 64 kDa
• Poids moléculaire observé: 75-85 kDa
• Numéro d’acquisition GenBank: BC131570
• Symbole du gène: CPT1B
• Identification du gène (NCBI): 1375
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Carnitine palmitoyltransferases (CPTs) mediate the transport of long chain fatty acyl groups into the mitochondrial matrix to undergo b-oxidation. Type I CPT resides in the outer mitochondrial membrane. Mammalian tissues express three isoforms: CPT1A (liver), CPT1B (muscle and heart), and CPT1C (brain). Several isoforms of CPT1B exist due to the alternative splicing, with predicted MW of 88/84/79/66 kDa.

Publications

Species	Application	Title
rat	WB	Cell Metab; A mechanosensitive lipolytic factor in the bone marrow promotes osteogenesis and lymphopoiesis.; Authors - Hui Peng
mouse	WB	Cell Metab; Global Analysis of Plasma Lipids Identifies Liver-Derived Acylcarnitines as a Fuel Source for Brown Fat Thermogenesis.; Authors - Judith Simcox ; KD Validated
mouse	WB	Nat Commun; Moderate-intensity interval exercise exacerbates cardiac lipotoxicity in high-fat, high-calories diet-fed mice; Authors - Jing Geng
human	WB	Adv Sci (Weinh); m6A-Modified circTET2 Interacting with HNRNPC Regulates Fatty Acid Oxidation to Promote the Proliferation of Chronic Lymphocytic Leukemia; Authors - Zijuan Wu
mouse	WB	Nat Commun; Mechanism of action and therapeutic route for a muscular dystrophy caused by a genetic defect in lipid metabolism.; Authors - Mahtab Tavasoli
human	WB	J Biomed Sci; Adipocyte pyroptosis occurs in omental tumor microenvironment and is associated with chemoresistance of ovarian cancer; Authors - Chang-Ni Lin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xhuljana (Verified Customer) (03-01-2024)

Tried in WB in C2C12 lysate, 30 *g

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C2C12 cells

CPT1B-specific Antibody Western Blot validation (1:1000 dilution) in C2C12 cells (Cat no:22170-1-AP)

FH

James (Verified Customer) (04-01-2021)

Green channel is CPT1B.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: lung

CPT1B-specific Antibody Immunofluorescence validation (1:100 dilution) in lung (Cat no:22170-1-AP)

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