Anticorps Polyclonal de lapin anti-CPT1C-specific

CPT1C-specific Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 12969-1-AP

Synonymes

CATL1, CPT IC, CPT1 B, CPT1C, CPT1C-specific, CPT1P, CPTI B, CPTIC

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Galerie de données de validation

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  WB analysis of mouse brain using 12969-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12969-1-AP (CPT1C-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 12969-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12969-1-AP (CPT1C-specific antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12969-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12969-1-AP (CPT1C-specific antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 12969-1-AP

  IP Result of anti-CPT1C-specific (IP:12969-1-AP, 5ug; Detection:12969-1-AP 1:500) with mouse testis tissue lysate 8000ug.

• 

  IHC staining of human gliomas using 12969-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12969-1-AP (CPT1C-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12969-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12969-1-AP (CPT1C-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 12969-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12969-1-AP (CPT1C-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 12969-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12969-1-AP (CPT1C-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 12969-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12969-1-AP (CPT1C-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 12969-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12969-1-AP (CPT1C-specific antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa, tissu cérébral de rat
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu de gliome humain, tissu cérébral humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IF: See 1 publications below

Informations sur le produit

12969-1-AP cible CPT1C-specific dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CPT1C-specific Protéine recombinante Ag3649
• Nom complet: carnitine palmitoyltransferase 1C
• Masse moléculaire calculée: 792 aa, 90 kDa
• Poids moléculaire observé: 70-82 kDa
• Numéro d’acquisition GenBank: BC029104
• Symbole du gène: CPT1C
• Identification du gène (NCBI): 126129
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CPT1C belongs to the carnitine/choline acetyltransferase family. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1C is an active forms of related brain-type carnitine palmitoyltransferase I. CPT1C may be a regulated target of malonyl-CoA that relays the "malonyl-CoA signal" in hypothalamic neurons that express the orexigenic and anorexigenic neuropeptides that regulate food intake and peripheral energy expenditure (PMID: 17018521). This antibody specifically recognizes CPT1C, and does not cross-react with CPT1A or CPT1B.

Publications

Species	Application	Title
human	WB	Cell Metab; Etomoxir Actions on Regulatory and Memory T Cells Are Independent of Cpt1a-Mediated Fatty Acid Oxidation.; Authors - Brenda Raud
human	WB	Cell Rep Med; Multiomic analysis identifies CPT1A as a potential therapeutic target in platinum-refractory, high-grade serous ovarian cancer.; Authors - Dongqing Huang
rat	WB	Proc Natl Acad Sci U S A; Important roles of brain-specific carnitine palmitoyltransferase and ceramide metabolism in leptin hypothalamic control of feeding.; Authors - Gao Su S
human	IF	JAMA Neurol; Mutation in CPT1C Associated With Pure Autosomal Dominant Spastic Paraplegia.; Authors - Carlo Rinaldi
human	WB	Cell Rep; HIF-1-Mediated Suppression of Acyl-CoA Dehydrogenases and Fatty Acid Oxidation Is Critical for Cancer Progression.; Authors - De Huang
rat	WB	FASEB J; Ghrelin effects on neuropeptides in the rat hypothalamus depend on fatty acid metabolism actions on BSX but not on gender.; Authors - Lage Ricardo R

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