Anticorps Polyclonal de lapin anti-CPT2

• Phare
• Validé par KD/KO

CPT2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (4)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(57)

Avis (1)

N° de cat : 26555-1-AP

Synonymes

Carnitine O-palmitoyltransferase 2, mitochondrial, carnitine palmitoyltransferase 2, CPT II, CPTASE, EC:2.3.1.21

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Galerie de données de validation

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  WB analysis of LNCaP using 26555-1-AP

  WB result of CPT2 antibody (26555-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPT2 transfected LNCaP cells.

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  WB analysis using 26555-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26555-1-AP (CPT2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 26555-1-AP

  IP result of anti-CPT2 (IP:26555-1-AP, 4ug; Detection:26555-1-AP 1:300) with mouse liver tissue lysate 6000ug.

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  IHC staining of mouse heart using 26555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26555-1-AP (CPT2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 26555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26555-1-AP (CPT2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 26555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26555-1-AP (CPT2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 26555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26555-1-AP (CPT2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of C2C12 using 26555-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using 26555-1-AP (CPT2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules LNCaP, cellules MCF-7, cellules T-47D, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: tissu hépatique de souris,
• Résultats positifs en IHC: tissu cardiaque de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules C2C12,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 52 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 6 publications below

Informations sur le produit

26555-1-AP cible CPT2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, poisson-zèbre, porc, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CPT2 Protéine recombinante Ag24897
• Nom complet: carnitine palmitoyltransferase 2
• Masse moléculaire calculée: 74 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC002445
• Symbole du gène: CPT2
• Identification du gène (NCBI): 1376
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Carnitine palmitoyltransferase-1 and -2 (CPT1 and CPT2) are two genetically distinct mitochondrial membrane bound enzymes and play critical role in the regulation of FAO in normal cells. CPT2 is an ubiquitous protein and locates in the inner membrane of mitochondrial (PMID: 29437870). CPT2 is frequently down-regulated in primary ovarian serous carcinomas, which is significantly correlated with poor survival of ovarian cancer patients (PMID: 33486313). Down-regulation of CPT2 was a major cause of acylcarnitine accumulation and a common feature in mouse models of obesity- and NASH-driven HCC and human SH-HCC (PMID: 29872321).

Publications

Species	Application	Title
mouse	WB	Cell Metab; Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity; Authors - Zilong Zhang
rat	WB,IP	Cell Stem Cell; A small-molecule cocktail promotes mammalian cardiomyocyte proliferation and heart regeneration.; Authors - Jianyong Du
mouse	WB	Nat Commun; Hyodeoxycholic acid ameliorates nonalcoholic fatty liver disease by inhibiting RAN-mediated PPARα nucleus-cytoplasm shuttling; Authors - Jing Zhong
mouse	WB	Nat Commun; Moderate-intensity interval exercise exacerbates cardiac lipotoxicity in high-fat, high-calories diet-fed mice; Authors - Jing Geng
human	WB	Dev Cell; Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation; Authors - Wanxin Guo
mouse	WB	Theranostics; Mitochondrial STAT3 exacerbates LPS-induced sepsis by driving CPT1a-mediated fatty acid oxidation.; Authors - Rongqing Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mitchell (Verified Customer) (09-26-2019)

Worked great for western blot in proximal intestine and liver.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: Intestine and Liver

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