Anticorps Polyclonal de lapin anti-CRABP1

CRABP1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 12588-1-AP

Synonymes

CRABP, CRABP I, CRABP1, CRABPI, RBP5

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Galerie de données de validation

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  WB analysis of mouse embryo using 12588-1-AP

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 12588-1-AP (CRABP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 12588-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 12588-1-AP (CRABP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 12588-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 12588-1-AP (CRABP1 Antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 12588-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12588-1-AP (CRABP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12588-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12588-1-AP (CRABP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu embryonnaire de souris, cellules HEK-293 transfectées, tissu splénique humain
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

12588-1-AP cible CRABP1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CRABP1 Protéine recombinante Ag3291
• Nom complet: cellular retinoic acid binding protein 1
• Masse moléculaire calculée: 137 aa, 15 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC022069
• Symbole du gène: CRABP1
• Identification du gène (NCBI): 1381
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The cellular retinoic acid-binding proteins including CRABP1 and CRABP2 bind retinoic acid (RA), an important regulator of cell growth and differentiation, thus play an important role in RA-mediated differentiation and proliferation processes. It has been reported that CRABP1 influences the biological effects of RA in cell-selective manners by enhancing the physiological function of RA in keratinocytes or inhibiting RA-induced differentiation of neuroblastoma cells.

Publications

Species	Application	Title
chicken	IF	Elife; Single-cell proteomics reveals changes in expression during hair-cell development.; Authors - Ying Zhu
human	WB,IHC	Mol Oncol; CRABP-II methylation: a critical determinant of retinoic acid resistance of medulloblastoma cells.; Authors - Fu Yuan-Shan YS
human	WB	Cell Rep; Greater epithelial ridge cells are the principal organoid-forming progenitors of the mouse cochlea.; Authors - Marie Kubota
mouse	WB	Virol J; All-trans retinoic acid increases the pathogenicity of the H9N2 influenza virus in mice; Authors - Xiaofei Niu
		Cell Rep; Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regeneration; Authors - Lixiang Sun