Anticorps Polyclonal de lapin anti-RAIDD

• Phare
• Validé par KD/KO

RAIDD Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 10401-1-AP

Synonymes

CRADD, RAIDD

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Galerie de données de validation

• 

  WB analysis of K-562 using 10401-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10401-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10401-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10401-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10401-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10401-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10401-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10401-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10401-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10401-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 10401-1-AP

  IP Result of anti-RAIDD (IP:10401-1-AP, 3ug; Detection:10401-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human colon cancer using 10401-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10401-1-AP (RAIDD antibody) at dilution of 1:100 (under 25x lens).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HEK-293, cellules HeLa, cellules MCF-7, cellules Raji, tissu cardiaque de souris, tissu de muscle squelettique de souris, tissu hépatique de souris, tissu rénal de souris, tissu testiculaire de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 5 publications below

Informations sur le produit

10401-1-AP cible RAIDD dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAIDD Protéine recombinante Ag0630
• Nom complet: CASP2 and RIPK1 domain containing adaptor with death domain
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC017042
• Symbole du gène: CRADD
• Identification du gène (NCBI): 8738
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAIDD, also named as CRADD, is an apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex. CRADD interacts with BCL10 through its caspase recruitment domain and suppresses interactions between BCL10 and CARMA1. It is a negative regulator of the CARMA1 signalosome and suppressor of Th1-and Th17-mediated inflammatory responses.

Publications

Species	Application	Title
mouse	WB	Dev Cell; E2F-Family Members Engage the PIDDosome to Limit Hepatocyte Ploidy in Liver Development and Regeneration.; Authors - Valentina C Sladky ; KD Validated
mouse	WB	EMBO Rep; PIDDosome-induced p53-dependent ploidy restriction facilitates hepatocarcinogenesis.; Authors - Valentina C Sladky
mouse	WB	J Immunol; Cutting edge: the "death" adaptor CRADD/RAIDD targets BCL10 and suppresses agonist-induced cytokine expression in T lymphocytes.; Authors - Lin Qing Q ; KO Validated
human, mouse	WB	J Biol Chem; The Adaptor CRADD/RAIDD Controls Activation of Endothelial Cells by Proinflammatory Stimuli.; Authors - Huan Qiao
mouse	WB	Cell Metab; PIDDosome-SCAP crosstalk controls high-fructose-diet-dependent transition from simple steatosis to steatohepatitis; Authors - Ju Youn Kim ; KO Validated