Anticorps Polyclonal de lapin anti-CRAT

• Phare
• Validé par KD/KO

CRAT Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 15170-1-AP

Synonymes

CACP, Carnitine acetylase, carnitine acetyltransferase, Carnitine O acetyltransferase, CAT, CAT1, CRAT

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Galerie de données de validation

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  WB analysis using 15170-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15170-1-AP (CRAT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 15170-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15170-1-AP (CRAT antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 15170-1-AP

  IP Result of anti-CRAT (IP:15170-1-AP, 4ug; Detection:15170-1-AP 1:300) with mouse skeletal muscle tissue lysate 3600ug.

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cœur de rat, cœur de souris, muscle squelettique de rat, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu de muscle squelettique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below

Informations sur le produit

15170-1-AP cible CRAT dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CRAT Protéine recombinante Ag7422
• Nom complet: carnitine acetyltransferase
• Masse moléculaire calculée: 71 kDa
• Poids moléculaire observé: 55-68 kDa
• Numéro d’acquisition GenBank: BC000723
• Symbole du gène: CRAT
• Identification du gène (NCBI): 1384
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CRAT, also named as CAT1, belongs to the carnitine/choline acetyltransferase family. It is specific for short chain fatty acids. CRAT seems to affect the flux through the pyruvate dehydrogenase complex. It may be involved as well in the transport of acetyl-CoA into mitochondria. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CRAT is an active forms for carnitine acetyltransferase. This antibody can bind the close sequences genes.

Publications

Species	Application	Title
rat	WB	Cell Metab; Dissociation of Muscle Insulin Resistance from Alterations in Mitochondrial Substrate Preference.; Authors - Joongyu D Song
		Cancer Res; Organelle-Derived Acetyl-CoA Promotes Prostate Cancer Cell Survival, Migration, and Metastasis via Activation of Calmodulin Kinase II.; Authors - Guoyu Yu
goat	WB	Redox Biol; TGF-β1 attenuates mitochondrial bioenergetics in pulmonary arterial endothelial cells via the disruption of carnitine homeostasis.; Authors - Xutong Sun ; KD Validated
sheep	WB	Antioxid Redox Signal; Disruption of endothelial cell mitochondrial bioenergetics in lambs with increased pulmonary blood flow.; Authors - Sun Xutong X
mouse	WB	Mol Metab; Carnitine acetyltransferase (CRAT) expression in macrophages is dispensable for nutrient stress sensing and inflammation.; Authors - Emily L Goldberg
mouse	WB	Heliyon; Stable isotope-labeled carnitine reveals its rapid transport into muscle cells and acetylation during contraction; Authors - Yasuro Furuichi