Anticorps Polyclonal de lapin anti-CREB3L1,OASIS

• Phare
• Validé par KD/KO

CREB3L1,OASIS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(12)

N° de cat : 11235-2-AP

Synonymes

CREB3L1, OASIS, Processed cyclic AMP-responsive element-binding protein 3-like protein 1, Old astrocyte specifically-induced substance, Cyclic AMP-responsive element-binding protein 3-like protein 1

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Galerie de données de validation

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  WB analysis of A549 using 11235-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This batch of A549 cells lysate predominantly expressed the maturer form likely due to cell stage.

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  WB analysis of COLO 320 using 11235-2-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11235-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This batch of A549 cells lysate predominantly expressed the precursor form likely due to cell stage.

• 

  IHC staining of human prostate cancer using 11235-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:50 (under 25x lens).

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  IF Staining of A549 using 11235-2-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules COLO 320
• Résultats positifs en IHC: tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

11235-2-AP cible CREB3L1,OASIS dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CREB3L1,OASIS Protéine recombinante Ag1757
• Nom complet: cAMP responsive element binding protein 3-like 1
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 55-58 kDa and 84 kDa
• Numéro d’acquisition GenBank: BC015781
• Symbole du gène: CREB3L1/OASIS
• Identification du gène (NCBI): 90993
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cyclic AMP-responsive element-binding protein 3-like protein 1(CREB3L1) belongs to a family of transcription factors that are synthesized as membrane-bound precursors in the endoplasmic reticulum (ER) and then transported to the Golgi where they are activated through regulated intramembrane proteolysis (RIP). Also it participates in ER-stress response in astrocytes in the central nervous system. It may has a role in gliosis, and involves in activation of transcription through box-B element but not CRE. CREB3L1 acts as a transcription repressor in proliferation of virus-infected cell. The full-length precursor form (approximately 84 kDa) of CREB3L1 and the matured form of CREB3L1 is 58 kDa. (PMID: 20668028)

Publications

Species	Application	Title
	WB	Epigenetics; OASIS/CREB3L1 is epigenetically silenced in human bladder cancer facilitating tumor cell spreading and migration in vitro.; Authors - Michael Rose
rat	WB	Endocrinology; OASIS/CREB3L1 induces expression of genes involved in extracellular matrix production but not classical endoplasmic reticulum stress response genes in pancreatic beta-cells.; Authors - Vellanki Ravi N RN
human	WB,IHC	Breast Cancer Res; Epigenetic silencing of CREB3L1 by DNA methylation is associated with high-grade metastatic breast cancers with poor prognosis and is prevalent in triple negative breast cancers.; Authors - Alison K Ward
human, rat	WB	PLoS One; OASIS/CREB3L1 is induced by endoplasmic reticulum stress in human glioma cell lines and contributes to the unfolded protein response, extracellular matrix production and cell migration.; Authors - Vellanki Ravi N RN ; KD Validated
human	WB,IHC	BMC Cancer; CREB3L1 as a potential biomarker predicting response of triple negative breast cancer to doxorubicin-based chemotherapy.; Authors - Bray Denard
rat	WB	Front Endocrinol (Lausanne); Maternal Protein Restriction Induces Alterations in Hepatic Unfolded Protein Response-Related Molecules in Adult Rat Offspring.; Authors - Xiaomei Liu

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