Anticorps Polyclonal de lapin anti-CREB3L2

CREB3L2 Polyclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 14514-1-AP

Synonymes

BBF2H7, CREB3L2

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Galerie de données de validation

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  WB analysis of HepG2 using 14514-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14514-1-AP (CREB3L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14514-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14514-1-AP (CREB3L2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 14514-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14514-1-AP (CREB3L2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 14514-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 14514-1-AP (CREB3L2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, tissu hépatique de souris, tissu testiculaire de souris
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

14514-1-AP cible CREB3L2 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CREB3L2 Protéine recombinante Ag5973
• Nom complet: cAMP responsive element binding protein 3-like 2
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC063666
• Symbole du gène: CREB3L2
• Identification du gène (NCBI): 64764
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cyclic AMP-responsive element-binding protein 3-like protein 2 (CREB3L2), also named BBF2H7, is a transcriptional activator which is activated during endoplasmic reticulum (ER) stress late phase. It has 3 isoforms produced by alternative splicing. CREB3L2 exerts protective effect in neurons by regulation of unfolded protein response (UPR)-dependent gene transcriptions. N-terminal cytoplamic domain of CREB3L2 is cleaved under ER stress and then translocates to the nucleus. Catalog#14514-1-AP is a rabbit polyclonal antibody raised against N-terminal (1-247aa) of human CREB3L2.

Publications

Species	Application	Title
human	WB	Br J Pharmacol; Azithromycin alleviates the severity of rheumatoid arthritis by targeting the unfolded protein response component of glucose-regulated protein 78 (GRP78).; Authors - Yongli Zhang
human	WB	Int J Mol Sci; Comparative Analysis of CREB3 and CREB3L2 Protein Expression in HEK293 Cells.; Authors - Kentaro Oh-Hashi
human	WB,IF	J Invest Dermatol; BBF2H7-mediated Sec23A pathway is required for endoplasmic reticulum-to-Golgi trafficking in dermal fibroblasts to promote collagen synthesis.; Authors - Ishikura-Kinoshita Seiko S
human	WB	J Biol Chem; APOBEC3G inhibits microRNA-mediated repression of translation by interfering with the interaction between Argonaute-2 and MOV10.; Authors - Liu Chao C
human	WB	Mol Biol Rep; Expression analysis and functional characterization of thioredoxin domain-containing protein 11; Authors - Ryoichi Murase