Anticorps Polyclonal de lapin anti-CREG1

CREG1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 12220-1-AP

Synonymes

CREG, CREG1, Protein CREG1

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Galerie de données de validation

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  WB analysis using 12220-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12220-1-AP (CREG1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12220-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12220-1-AP (CREG1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules K-562, tissu splénique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

12220-1-AP cible CREG1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CREG1 Protéine recombinante Ag2901
• Nom complet: cellular repressor of E1A-stimulated genes 1
• Masse moléculaire calculée: 220 aa, 24 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC008628
• Symbole du gène: CREG1
• Identification du gène (NCBI): 8804
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

REG1 (Cellular repressor of E1A-stimulated genes 1) shares limited sequence similarity with E1A and binds both the general transcription factor TBP and the tumor suppressor pRb in vitro(PMID:9710587). The monomers of CREG can associate into dimers and the dimerization is observed not only in the crystal but also in solution(PMID:16344469).The cellular repressor of E1A-stimulated genes (CREG) is a secreted glycoprotein that inhibits cell proliferation and/or enhances differentiation. CREG is widely expressed in adult tissues such as the brain, heart, lungs, liver, intestines and kidneys in mice(PMID:20857207).

Publications

Species	Application	Title
human	WB	Nature; A CRISPR screen defines a signal peptide processing pathway required by flaviviruses.; Authors - Rong Zhang
mouse	WB	Int J Mol Sci; TLR4-Mediated Inflammatory Responses Regulate Exercise-Induced Molecular Adaptations in Mouse Skeletal Muscle.; Authors - Haruna Fujiyoshi
human	WB	Carcinogenesis; PABPN1 Promotes clear cell Renal Cell Carcinoma progression by suppressing the alternative polyadenylation of SGPL1 and CREG1; Authors - Ming Xiong
rat	WB	J Cell Mol Med; Exercise ameliorates insulin resistance and improves ASK1-mediated insulin signalling in obese rats.; Authors - Yong Zhang
mouse	WB,IHC	J Biochem; CREG1 administration stimulates BAT thermogenesis and improves diet-induced obesity in mice.; Authors - Tatsuya Kusudo ; KD Validated
		FASEB J; CREG1 stimulates brown adipocyte formation and ameliorates diet-induced obesity in mice.; Authors - Michihiro Hashimoto