Anticorps Polyclonal de lapin anti-CREM

CREM Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 12131-1-AP

Synonymes

cAMP-responsive element modulator, hCREM 2, ICER

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Galerie de données de validation

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  WB analysis using 12131-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12131-1-AP (CREM antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 12131-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 12131-1-AP (CREM Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 12131-1-AP

  IP result of anti-CREM (IP:12131-1-AP, 4ug; Detection:12131-1-AP 1:300) with mouse testis tissue lysate 1800ug.

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  IHC staining of human prostate cancer using 12131-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12131-1-AP (CREM antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 12131-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12131-1-AP (CREM antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de rat, tissu de thymus de rat, tissu de thymus de souris
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Informations sur le produit

12131-1-AP cible CREM dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CREM Protéine recombinante Ag2775
• Nom complet: cAMP responsive element modulator
• Masse moléculaire calculée: 332 aa, 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC017117
• Symbole du gène: CREM
• Identification du gène (NCBI): 1390
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cyclic AMP-responsive element modulator (CREM) is a transcription factor highly expressed in the post-meiotic germ cells of the testis. Its pivotal role is to regulate the expression of several germ cell-specific genes, a crucial function as demonstrated by the severe phenotype of mice whose CREM gene was mutated by homologous recombination. CREM-deficient male animals are sterile and display a ten-fold increase in the apoptosis of germ cells. Recent results have shown that CREM needs a tissue-specific co-activator, ACT (activator of CREM in testis) to elicit its regulatory function in testis (PMID: 10824972). Also, CREM is a crucial regulator of NK cell function. CREM exerts its regulatory functions through epigenetic reprogramming of CAR-NK cells (PMID: 40468083).

Publications

Species	Application	Title
human	WB	Nature; A druggable copper-signalling pathway that drives inflammation; Authors - Stéphanie Solier
mouse	IF	Biofabrication; Testis on a chip - a microfluidic 3-dimensional culture system for the development of spermatogenesisin-vitro.; Authors - Ali AbuMadighem
mouse	IF	Int J Mol Sci; Effect of Chemotherapy Cytarabine and Acute Myeloid Leukemia on the Development of Spermatogenesis at the Adult Age of Immature Treated Mice.; Authors - Bara'ah Khaleel
mouse	IF	Int J Mol Sci; The Expression Levels and Cellular Localization of Pigment Epithelium Derived Factor (PEDF) in Mouse Testis: Its Possible Involvement in the Differentiation of Spermatogonial Cells.; Authors - Noy Bagdadi
mouse	IF	Int J Mol Sci; Effect of Temperature on the Development of Stages of Spermatogenesis and the Functionality of Sertoli Cells In Vitro; Authors - Areej Jorban
	IF	Life Sci; Adrenocorticotropic hormone and its receptor as a novel testicular system involves in the development of spermatogenesis; Authors - AbuMadighem Ali

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