Anticorps Polyclonal de lapin anti-CREST

• Phare
• Validé par KD/KO

CREST Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(11)

Avis (1)

N° de cat : 12439-1-AP

Synonymes

SS18L1, Calcium-responsive transactivator, KIAA0693, LP2261, SS18 like protein 1

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Galerie de données de validation

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  WB analysis using 12439-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12439-1-AP (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12439-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12439-1-AP (CREST antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12439-1-AP

  WB result of CREST antibody (12439-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CREST transfected HeLa cells.

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  WB analysis of COLO 320 using 12439-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12439-1-AP (CREST antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 12439-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12439-1-AP (CREST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12439-1-AP

  IP result of anti-CREST (IP:12439-1-AP, 4ug; Detection:12439-1-AP 1:300) with HeLa cells lysate 3000 ug.

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  IP experiment of mouse brain using 12439-1-AP

  IP result of anti-CREST (IP:12439-1-AP, 3ug; Detection:12439-1-AP 1:1000) with mouse brain tissue lysate 6000ug.

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  IHC staining of human breast cancer using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse testis using 12439-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12439-1-AP (CREST antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse testis using 12439-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12439-1-AP (CREST antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 12439-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CREST antibody (12439-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules COLO 320, cellules HeLa, tissu cardiaque humain, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules HeLa, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu testiculaire de souris,
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

12439-1-AP cible CREST dans les applications de WB, IHC, IF/ICC, IF-P, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Drosophile, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CREST Protéine recombinante Ag3119
• Nom complet: synovial sarcoma translocation gene on chromosome 18-like 1
• Masse moléculaire calculée: 396 aa, 43 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC034494
• Symbole du gène: CREST
• Identification du gène (NCBI): 26039
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CREST, also named as SS18-like 1(SS18L1) is a transcriptional activator that is required for calcium-dependent dendritic growth and branching in cortical neurons. It's also a nuclear protein interacts with CREB-binding protein and expressed in the developing brain. It helps regulate neuronal morphogenesis in calcuim-dependent manner. The N-terminal domain of SS18L1 is required for suppressing transactivation in the basal state, while the C-terminal domain is required for calcium-induced transactivation. It's part of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. This complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. The calculated molecular weight of CREST is about 43 kDa, but the modified of CREST protein is 55 kDa (PMID: 25888396). CREST exists some isoforms with calulated MV 43, 40, 33 and 29 kDa.

Publications

Species	Application	Title
mouse,human	IHC,CoIP	Nat Neurosci; Exome sequencing to identify de novo mutations in sporadic ALS trios.; Authors - Chesi Alessandra A
		Nat Commun; Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2.; Authors - Haining Zhou
mouse	IF	J Neurosci; Kinetic Analysis of npBAF to nBAF Switching Reveals Exchange of SS18 with CREST and Integration with Neural Developmental Pathways.; Authors - Staahl Brett T BT
human	ChIP	Cancers (Basel); Global Chromatin Changes Resulting from Single-Gene Inactivation-The Role of SMARCB1 in Malignant Rhabdoid Tumor.; Authors - Colin Kenny
rat	WB,IF	J Neurosci; CREST in the Nucleus Accumbens Core Regulates Cocaine Conditioned Place Preference, Cocaine-Seeking Behavior, and Synaptic Plasticity.; Authors - Yasaman Alaghband
Drosophila	WB	PLoS Genet; Calcium-responsive transactivator (CREST) toxicity is rescued by loss of PBP1/ATXN2 function in a novel yeast proteinopathy model and in transgenic flies.; Authors - Sangeun Park

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aktan (Verified Customer) (12-24-2019)

The product is tested in prostate cancer cell lines. Used at the given dilution factor, in 5% BSA in PBST, o/n at 4C. Bands were detected using Licor system and Licor near IR secondary antibodies.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Prostate cancer cell lines

CREST Antibody Western Blot, validation (1:500 dilution) in Prostate cancer cell lines (Cat no:12439-1-AP)

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