Anticorps Polyclonal de lapin anti-CRHR1

CRHR1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(13)

N° de cat : 20967-1-AP

Synonymes

CRF R, CRF R 1, CRF R1, CRF1, CRF1R, CRFR, CRFR 1, CRFR1, CRH R 1, CRH R1, CRH R1h, CRHR, CRHR1, CRHR1f

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Galerie de données de validation

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  WB analysis of HeLa using 20967-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20967-1-AP (CRHR1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 20967-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20967-1-AP (CRHR1 Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IF: See 4 publications below

Informations sur le produit

20967-1-AP cible CRHR1 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: corticotropin releasing hormone receptor 1
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 46-55 kDa
• Numéro d’acquisition GenBank: NM_004382
• Symbole du gène: CRHR1
• Identification du gène (NCBI): 1394
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Corticotropin-releasing hormone (CRH, also known as Corticotropin-releasing factor, CRF) is a 41 amino acid peptide hormone and a key regulator of the stress response. CRH and the related urocortin peptides (UCN, UCN2 and UCN3) mediate their actions through two mammalian receptor subtypes, CRHR1 and CRHR2 (PMID: 23863939). CRHR1 (also known as CRFR1) exhibits high affinity towards CRH and UCN but low affinity towards UCN2 and no affinity towards UCN3 (PMID: 22684850). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Widely distributed throughout the brain, CRHR1 critically controls behavioral adaptation to stress and is causally linked to emotional disorders (PMID: 21885734). Alternative splicing results in multiple transcript variants encoding distinct isoforms of CRHR1. This antibody raised against a synthesized peptide corresponding to amino acid residues 148-165 of isoform CRF-R1 can only recognize isoform CRF-R1.

Publications

Species	Application	Title
	IF	Proc Natl Acad Sci U S A; Overactivation of corticotropin-releasing factor receptor type 1 and aquaporin-4 by hypoxia induces cerebral edema.; Authors - Shao-Jun Chen
human	WB,IF	Pharmaceuticals (Basel); A Novel Mast Cell Stabilizer JM25-1 Rehabilitates Impaired Gut Barrier by Targeting the Corticotropin-Releasing Hormone Receptors; Authors - Yueshan Sun
	WB	Behav Brain Res; Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats.; Authors - Shuai Lian
	WB	Brain Res; Response of the maternal hypothalamus to cold stress during late pregnancy in rats.; Authors - Di Wang
	WB	Int J Mol Med; Sini San ameliorates duodenal mucosal barrier injury and low‑grade inflammation via the CRF pathway in a rat model of functional dyspepsia.; Authors - Chunyang Zhu
	WB	Reprod Toxicol; Dynamic expression of corticotropin-releasing hormone and urocortin in estrogen induced-cholestasis pregnant rat.; Authors - Fan Zhou

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