Anticorps Polyclonal de lapin anti-CRM1

CRM1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (2)

N° de cat : 27917-1-AP

Synonymes

CRM1, Exp1, Exportin 1, XPO1

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Galerie de données de validation

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  WB analysis using 27917-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27917-1-AP (CRM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 27917-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 27917-1-AP (CRM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27917-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27917-1-AP (CRM1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 27917-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27917-1-AP (CRM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 27917-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27917-1-AP (CRM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules C6, cellules HeLa, cellules HT-1080, cellules K-562, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

27917-1-AP cible CRM1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CRM1 Protéine recombinante Ag27207
• Nom complet: exportin 1 (CRM1 homolog, yeast)
• Masse moléculaire calculée: 1071 aa, 123 kDa
• Poids moléculaire observé: 123 kDa
• Numéro d’acquisition GenBank: BC032847
• Symbole du gène: XPO1
• Identification du gène (NCBI): 7514
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CRM1 (chromosome region maintenance 1, also known as exportin-1 or XPO1), a member of the karyopherin β family of transport receptors, is an important nuclear export receptor recognizing proteins bearing a leucine-rich nuclear export signal (PMID: 9323133). CRM1 is the major receptor for the export of proteins out of the nucleus and is also required for transport of many RNAs (PMID: 17317185). In addition to nuclear export, CRM1 also plays a role in centrosome duplication and spindle assembly (PMID: 22773955). Elevated CRM1 expression has been reported in several cancers, which implicates CRM1 as a potential anti-cancer drug target (PMID: 22677130; 21683812 ).

Publications

Species	Application	Title
human	IF	J Cell Commun Signal; CD47 interactions with exportin-1 limit the targeting of m 7 G-modified RNAs to extracellular vesicles; Authors - Sukhbir Kaur
mouse	IHC	J Cancer Res Clin Oncol; Selinexor demonstrates anti-tumor efficacy in paired patient-derived xenograft models and hydrogel-embedded histoculture drug sensitivity test of penile cancer; Authors - Yuanqiao He
human	WB	Mol Cell; EGFR promotes ALKBH5 nuclear retention to attenuate N6-methyladenosine and protect against ferroptosis in glioblastoma; Authors - Deguan Lv
mouse	WB	Mol Cell; A GAPDH serotonylation system couples CD8+ T cell glycolytic metabolism to antitumor immunity; Authors - Xu Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xingyuan (Verified Customer) (07-17-2023)

The band quality was pretty good

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293T

CRM1 Antibody Western Blot validation (1:5000 dilution) in HEK293T (Cat no:27917-1-AP)

FH

Wei (Verified Customer) (03-12-2020)

strong, clear band for mouse heart lysates

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart