Anticorps Polyclonal de lapin anti-CRMP1

CRMP1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 10317-1-AP

Synonymes

CRMP 1, CRMP1, DPYSL1, DRP 1, DRP1, ULIP 3, ULIP3, Unc 33 like phosphoprotein 3

---

Galerie de données de validation

• 

  WB analysis using 10317-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10317-1-AP (CRMP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10317-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10317-1-AP (CRMP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 10317-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10317-1-AP (CRMP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 10317-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10317-1-AP (CRMP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 10317-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10317-1-AP (CRMP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10317-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10317-1-AP (CRMP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 10317-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CRMP1 antibody (10317-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of U2OS using 10317-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CRMP1 antibody (10317-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, tissu cérébral de souris, tissu pulmonaire de rat
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

10317-1-AP cible CRMP1 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CRMP1 Protéine recombinante Ag0369
• Nom complet: collapsin response mediator protein 1
• Masse moléculaire calculée: 62 kDa
• Poids moléculaire observé: 62 kDa
• Numéro d’acquisition GenBank: BC000252
• Symbole du gène: CRMP1
• Identification du gène (NCBI): 1400
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CRMP1 (Collapsin response mediator protein 1) is also named as DPYSL1, ULIP3, DRP-1 and belongs to the collapsin response mediator protein family, which are a family of five cytoplasmic proteins predominantly expressed in the developing nervous system. CRMP1, the predicted 62-kDa protein and its 75-kDa isoform corresponding to its N-terminal splice variant already described for embryonic, neonatal, and adult brain of the rat are evidenced in the cortex of adult mouse (PMID:15834957). This protein is involved in invasion, and aggressiveness of prolactin pituitary tumors.

Publications

Species	Application	Title
human	WB	Chem Biol Interact; Exposure to Benzo(a)pyrene damages mitochondrial function via suppressing mitochondrial melatonin receptors in ovarian corpus luteum during early pregnancy; Authors - Jie Yang
rat	WB	Molecules; Senescent Phenotype of Astrocytes Leads to Activation of BV2 Microglia and N2a Neuronal Cells Death; Authors - Wenyou Zhang
mouse,human	WB,CoIP	Neuron; Species-specific FMRP regulation of RACK1 is critical for prenatal cortical development; Authors - Minjie Shen