Anticorps Monoclonal anti-C-Reactive Protein/CRP

• Phare
• Validé par KD/KO

C-Reactive Protein/CRP Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris et plus (1)

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(12)

CloneNo.

3F10A11

N° de cat : 66250-1-Ig

Synonymes

CRP, PTX1, C-reactive protein(1-205), C-Reactive Protein, C reactive protein

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Galerie de données de validation

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  WB analysis of serum from mouse injected with bacteria using 66250-1-Ig

  Serum from mouse injected with bacteria were subjected to SDS PAGE followed by western blot with 66250-1-Ig (C-Reactive Protein/CRP Antibody) at dilution of 1:2000. Horseradish peroxidase conjugated protein A was used for signal developing instead of secondary antibody incubated at room temperature for 1.5 hours.

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  WB analysis of serum from mouse injected with bacteria using 66250-1-Ig

  Serum from normal mouse (left) and mouse injected with bacteria (right) were subjected to SDS PAGE followed by western blot with 66250-1-Ig (C-Reactive Protein/CRP Antibody) at dilution of 1:1000. Horseradish peroxidase conjugated protein A was used for signal developing instead of secondary antibody incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 66250-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66250-1-Ig (C-Reactive Protein/CRP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 66250-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66250-1-Ig (C-Reactive Protein/CRP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 66250-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66250-1-Ig (C-Reactive Protein/CRP Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 66250-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66250-1-Ig (C-Reactive Protein/CRP Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of human liver using 66250-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using 66250-1-Ig (CRP antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: sérum de souris injecté avec des bactéries, sérum de souris injecté avec le tissu bactérien, tissu hépatique de porc, tissu hépatique de rat
• Résultats positifs en IHC: tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu hépatique humain,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

66250-1-Ig cible C-Reactive Protein/CRP dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, singe, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: C-Reactive Protein/CRP Protéine recombinante Ag9883
• Nom complet: C-reactive protein, pentraxin-related
• Masse moléculaire calculée: 224 aa, 25 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC020766
• Symbole du gène: CRP
• Identification du gène (NCBI): 1401
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

C-reactive protein (CRP) is an acute-phase serum protein synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name of CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein that belongs to the pentraxin family of proteins. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation.

Publications

Species	Application	Title
rat	WB	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
human	IF	Front Immunol; Expression and clinical significance of interleukin-6 pathway in cholangiocarcinoma; Authors - Dongqing Gu
human	IHC	J Cell Mol Med; Proteomic studies in VWA1-related neuromyopathy allowed new pathophysiological insights and the definition of blood biomarkers; Authors - Mohammed Athamneh
mouse	WB	Front Immunol; Binding of Macrophage Receptor MARCO, LDL, and LDLR to Disease-Associated Crystalline Structures.; Authors - Anika Alberts
		Sci Rep; A label-free fiber optic SPR biosensor for specific detection of C-reactive protein.; Authors - Wenjia Wang
human	WB	Sci Rep; C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.; Authors - Anika Alberts

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