Anticorps Polyclonal de lapin anti-CRTAP

• Phare
• Validé par KD/KO

CRTAP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 15724-1-AP

Synonymes

Cartilage-associated protein, CASP, LEPREL3

---

Galerie de données de validation

• 

  WB analysis using 15724-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15724-1-AP

  WB result of CRTAP antibody (15724-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CRTAP transfected HeLa cells.

• 

  WB analysis of HeLa using 15724-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15724-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 15724-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 15724-1-AP

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 15724-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15724-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 15724-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 15724-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 15724-1-AP

  IP result of anti-CRTAP (IP:15724-1-AP, 4ug; Detection:15724-1-AP 1:500) with HEK-293 cells lysate 3000 ug.

• 

  IHC staining of human kidney using 15724-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15724-1-AP (CRTAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15724-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15724-1-AP (CRTAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15724-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CRTAP antibody (15724-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin was stained using CL594-phalloidin (red) and DNA was stained by DAPI (blue).

---

Applications testées

• Résultats positifs en WB: cellules HT-1080, cellules HEK-293, cellules HeLa, cellules NIH/3T3, cellules ROS1728, tissu de côlon humain, tissu d'estomac humain
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

15724-1-AP cible CRTAP dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CRTAP Protéine recombinante Ag8459
• Nom complet: cartilage associated protein
• Masse moléculaire calculée: 46.5 kDa
• Poids moléculaire observé: 43-47 kDa
• Numéro d’acquisition GenBank: BC008745
• Symbole du gène: CRTAP
• Identification du gène (NCBI): 10491
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IHC	Med Oncol; Data-independent acquisition (DIA) mass spectrometry reveals related proteins involved in the occurrence of early intestinal-type gastric cancer; Authors - Liangshun Zhang
human	WB	Nat Commun; The structural basis for the collagen processing by human P3H1/CRTAP/PPIB ternary complex; Authors - Wenguo Li

{{ptg:RelatedPrimaryAntibodies}}