Anticorps Polyclonal de lapin anti-CRX

CRX Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(4)

Avis (1)

N° de cat : 12047-1-AP

Synonymes

cone rod homeobox, Cone rod homeobox protein, CORD2, CRD, CRX, diencephalon/mesencephalon homeobox 1, DMBX1, LCA7, OTX3

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Galerie de données de validation

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  WB analysis of Y79 using 12047-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12047-1-AP (CRX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 12047-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12047-1-AP (CRX antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of Y79 using 12047-1-AP

  IP result of anti-CRX (IP:12047-1-AP, 4ug; Detection:12047-1-AP 1:500) with Y79 cells lysate 1920ug.

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  IHC staining of mouse eye using 12047-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 12047-1-AP (CRX antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 12047-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12047-1-AP (CRX antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Y79
• Résultats positifs en IP: cellules Y79
• Résultats positifs en IHC: tissu oculaire de souris, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

12047-1-AP cible CRX dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CRX Protéine recombinante Ag2677
• Nom complet: cone-rod homeobox
• Masse moléculaire calculée: 299 aa, 32 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC016664
• Symbole du gène: CRX
• Identification du gène (NCBI): 1406
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cone-rod homeobox (Crx) is a homeodomain transcription factor and member of the Otx family, which has been thought to play a critical role in determining and maintaining the phenotype of both pinealocytes and retinal photoreceptors [PMID:17467693]. In the mammalian retina, Crx plays an essential role in the normal development and maintenance of cones and rods and regulates expression of the network of genes that characterize the retina [PMID:17653270]. Elimination of Crx by disruption of the homeobox domain results in loss of the image-forming visual system but not the non-image-forming visual system controlling circadian rhythms [PMID:20438719].

Publications

Species	Application	Title
human	WB, CoIP	J Neurosci; The transcription factor GTF2IRD1 regulates the topology and function of photoreceptors by modulating photoreceptor gene expression across the retina.; Authors - Tomohiro Masuda
rat	IF	iScience; Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration; Authors - Haibin Tian
mouse	IHC	Dev Dyn; Reduced mTORC1-signaling in progenitor cells leads to retinal lamination deficits; Authors - Christoffer Nord
rat	IF	Neural Regen Res; Müller cells are activated in response to retinal outer nuclear layer degeneration in rats subjected to simulated weightlessness conditions; Authors - Yuxue Mu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christoffer (Verified Customer) (03-03-2025)

Works with paraffin embedded adult mouse retina.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/200
• Cell Tissue Type: Adult mouse retina.

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