Anticorps Polyclonal de lapin anti-CSE1L

• Phare
• Validé par KD/KO

CSE1L Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 22219-1-AP

Synonymes

CAS, CSE1, CSE1L, Exp2, Exportin 2, Importin alpha re exporter, XPO2

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Galerie de données de validation

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  WB analysis using 22219-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22219-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22219-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22219-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22219-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 22219-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW264.7 using 22219-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 22219-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 22219-1-AP

  IP result of anti-CSE1L(IP:22219-1-AP, 4ug; Detection:22219-1-AP 1:2000) with HEK-293 cells lysate 1400 ug.

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  IHC staining of human placenta using 22219-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22219-1-AP (CSE1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 22219-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22219-1-AP (CSE1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 22219-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22219-1-AP (CSE1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 22219-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22219-1-AP (CSE1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, cellules MCF-7, cellules NIH/3T3, cellules Ramos, RAW264.7, tissu testiculaire de souris
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu placentaire humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

22219-1-AP cible CSE1L dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CSE1L Protéine recombinante Ag17556
• Nom complet: CSE1 chromosome segregation 1-like (yeast)
• Masse moléculaire calculée: 971 aa, 110 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC109313
• Symbole du gène: CSE1L
• Identification du gène (NCBI): 1434
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CSE1L (Chromosome Segregation 1-Like) is located on 20q13.13, and encodes a 971-amino-acid protein. It associates with microtubules and mitotic spindles, and is also considered to have a role in tumor progression. The CSE1L protein includes a nuclear localization signal allowing for transport into the nucleus via the importin-alpha/beta heterodimer. In addition, CSE1L plays roles in cell proliferation and apoptosis.

Publications

Species	Application	Title
human,mouse	WB,IHC	Nat Commun; Genome-wide enhancer-gene regulatory maps link causal variants to target genes underlying human cancer risk; Authors - Pingting Ying
human	WB	Cancers (Basel); GDF15 Is an Eribulin Response Biomarker also Required for Survival of DTP Breast Cancer Cells.; Authors - Chiara Bellio
human	WB,IHC	Sci Rep; CSE1L interaction with MSH6 promotes osteosarcoma progression and predicts poor patient survival.; Authors - Dong-Dong Cheng ; KD Validated
human	WB	J Biol Chem; RNA-binding proteins and heat-shock protein 90 are constituents of the cytoplasmic capping enzyme interactome.; Authors - Jackson B Trotman
human,mouse	WB,IHC,IF	Int J Oncol; Butyrate supplementation regulates expression of chromosome segregation 1‑like protein to reverse the genetic distortion caused by p53 mutations in colorectal cancer.; Authors - Chun-Chao Chang ; KD Validated
human	WB	J Cancer; CSE1L, as a novel prognostic marker, promotes pancreatic cancer proliferation by regulating the AKT/mTOR signaling pathway.; Authors - Xiao Zhang ; KD Validated