Anticorps Polyclonal de lapin anti-Casein Kinase 1 Delta

• Phare
• Validé par KD/KO

Casein Kinase 1 Delta Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(17)

Avis (1)

N° de cat : 14388-1-AP

Synonymes

EC:2.7.11.26, EC:2.7.11.1, CSNK1D, CKI-delta, CKId

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Galerie de données de validation

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  WB analysis using 14388-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14388-1-AP (Casein Kinase 1 Delta antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14388-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14388-1-AP (Casein Kinase 1 delta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14388-1-AP

  IP result of anti-Casein Kinase 1 Delta (IP:14388-1-AP, 3ug; Detection:14388-1-AP 1:1000) with HeLa cells lysate 2000ug.

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  IHC staining of mouse brain using 14388-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14388-1-AP (Casein Kinase 1 Delta antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 14388-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14388-1-AP (Casein Kinase 1 Delta antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14388-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Casein Kinase 1 Delta antibody (14388-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of U-251 using 14388-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using Casein Kinase 1 Delta antibody (14388-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules K-562
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu cérébral de souris, tissu cardiaque de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U-251, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

14388-1-AP cible Casein Kinase 1 Delta dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Casein Kinase 1 Delta Protéine recombinante Ag5856
• Nom complet: casein kinase 1, delta
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC003558
• Symbole du gène: CK1 Delta
• Identification du gène (NCBI): 1453
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CSNK1D(casein kinase I isoform delta) is also named as HCKID, CKId and belongs to the casein kinase I subfamily. CKI is a ubiquitous enzyme which has been found in the cytosol, associated with membranes, and in the nucleus. It can phosphorylate a large number of cytosolic, membrane-associated, and nuclear proteins(PMID:8454611). CSNK1D is found to be associated generally with granulovacuolar bodies and tau-containing neurofibrillary tangles in AD, DS, PSP, PDC, PPND, and controls, and Pick bodies and ballooned neurons in PiD(PMID:10924763). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
		Mol Cancer; SPOP-mediated ubiquitination and degradation of PDK1 suppresses AKT kinase activity and oncogenic functions.; Authors - Qiwei Jiang
human	WB	Dev Cell; Mitotic ER Exit Site Disassembly and Reassembly Are Regulated by the Phosphorylation Status of TANGO1.; Authors - Miharu Maeda
human	WB	J Cell Biol; CHMP2B regulates TDP-43 phosphorylation and cytotoxicity independent of autophagy via CK1.; Authors - Xue Deng ; KD Validated
human	WB	Cell Chem Biol; CDK2 heterobifunctional degraders co-degrade CDK2 and cyclin E resulting in efficacy in CCNE1-amplified and overexpressed cancers; Authors - Nicholas Kwiatkowski
human	WB	Oncogene; β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.; Authors - Yanwei Lu ; KD Validated
human	WB	Mol Cell Biol; DNA damage regulates UHRF1 stability via the SCF(β-TrCP) E3 ligase.; Authors - Chen Hao H

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christina (Verified Customer) (11-28-2023)

I used the antibody to detect CKD1δ in WB after siRNA of CK1δ (MCF7 cells).

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: MCF7

Casein Kinase 1 Delta Antibody Western Blot validation (1:2000 dilution) in MCF7 (Cat no:14388-1-AP)

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