- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-Casein Kinase 1 Delta
Casein Kinase 1 Delta Polyclonal Antibody for IF, IP, WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris
Applications
WB, IP, IHC, IF, ELISA
Conjugaison
Non conjugué
N° de cat : 14388-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules HeLa, cellules HEK-293, cellules K-562 |
Résultats positifs en IP | cellules HeLa |
Résultats positifs en IF | cellules HepG2 |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF) | IF : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 2 publications below |
WB | See 6 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Informations sur le produit
14388-1-AP cible Casein Kinase 1 Delta dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Réactivité citée | Humain, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | Casein Kinase 1 Delta Protéine recombinante Ag5856 |
Nom complet | casein kinase 1, delta |
Masse moléculaire calculée | 47 kDa |
Poids moléculaire observé | 47 kDa |
Numéro d’acquisition GenBank | BC003558 |
Symbole du gène | CSNK1D |
Identification du gène (NCBI) | 1453 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
CSNK1D(casein kinase I isoform delta) is also named as HCKID, CKId and belongs to the casein kinase I subfamily. CKI is a ubiquitous enzyme which has been found in the cytosol, associated with membranes, and in the nucleus. It can phosphorylate a large number of cytosolic, membrane-associated, and nuclear proteins(PMID:8454611). CSNK1D is found to be associated generally with granulovacuolar bodies and tau-containing neurofibrillary tangles in AD, DS, PSP, PDC, PPND, and controls, and Pick bodies and ballooned neurons in PiD(PMID:10924763). It has 2 isoforms produced by alternative splicing.
Protocole
Product Specific Protocols | |
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WB protocol for Casein Kinase 1 Delta antibody 14388-1-AP | Download protocol |
IF protocol for Casein Kinase 1 Delta antibody 14388-1-AP | Download protocol |
IP protocol for Casein Kinase 1 Delta antibody 14388-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Cancer SPOP-mediated ubiquitination and degradation of PDK1 suppresses AKT kinase activity and oncogenic functions. | ||
Dev Cell Mitotic ER Exit Site Disassembly and Reassembly Are Regulated by the Phosphorylation Status of TANGO1. | ||
J Cell Biol CHMP2B regulates TDP-43 phosphorylation and cytotoxicity independent of autophagy via CK1.
| ||
Oncogene β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.
| ||
Cell Cycle Casein kinase 1 (α, δ and ε) localize at the spindle poles, but may not be essential for mammalian oocyte meiotic progression. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Christina (Verified Customer) (11-28-2023) | I used the antibody to detect CKD1δ in WB after siRNA of CK1δ (MCF7 cells).
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