Anticorps Polyclonal de lapin anti-CSNK2A1

• Phare
• Validé par KD/KO

CSNK2A1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

21

Avis (1)

N° de cat : 10992-1-AP

Synonymes

Casein kinase II subunit alpha, CK II alpha, CK2a, CK2A1, CK2α, CKII, CSNK2A1

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Galerie de données de validation

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  WB analysis using 10992-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10992-1-AP (CSNK2A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 10992-1-AP

  WB result of CSNK2A1 antibody (10992-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CSNK2A1 transfected PC-3 cells.

• 

  WB analysis using 10992-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10992-1-AP (CSNK2A1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10992-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10992-1-AP (CSNK2A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10992-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10992-1-AP (CSNK2A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10992-1-AP

  IP Result of anti-CSNK2A1 (IP:10992-1-AP, 4ug; Detection:10992-1-AP 1:1000) with HeLa cells lysate 2800ug.

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  IHC staining of human breast cancer using 10992-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10992-1-AP (CSNK2A1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10992-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10992-1-AP (CSNK2A1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10992-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10992-1-AP (CSNK2A1 antibody) at dilution of 1:200 (under 10x lens) proteolytic pre-treatment mediated antigen retrieved with Tris-EDTA buffer (pH9).

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  IHC staining of human colon cancer using 10992-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10992-1-AP (CSNK2A1 antibody) at dilution of 1:200 (under 40x lens) proteolytic pre-treatment mediated antigen retrieved with Tris-EDTA buffer (pH9).

• 

  IF Staining of HeLa using 10992-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10992-1-AP (CSNK2A1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Phalloidine stains the F-actin.

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules A431, cellules K-562, cellules NIH/3T3, cellules Raji
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 19 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

10992-1-AP cible CSNK2A1 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CSNK2A1 Protéine recombinante Ag1452
• Nom complet: casein kinase 2, alpha 1 polypeptide
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC011668
• Symbole du gène: CSNK2A1
• Identification du gène (NCBI): 1457
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. This enzyme is composed of an α subunit (37 to 44kDa) and a β subunit (24 to 28 kDa) and exists as an α2β2 tetramer (PMID:1448100). Casein kinase 2 has been found to be increased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes.

Publications

Species	Application	Title
human	WB,IHC	Adv Sci (Weinh); Inhibition of CK2/ING4 Pathway Facilitates Non-Small Cell Lung Cancer Immunotherapy; Authors - Qian Gou
human	WB	Nucleic Acids Res; CSNK2B modulates IRF1 binding to functional DNA elements and promotes basal and agonist-induced antiviral signaling; Authors - Moe Matsumoto ; KD Validated
human	WB	Nucleic Acids Res; CK2 kinase-mediated PHF8 phosphorylation controls TopBP1 stability to regulate DNA replication.; Authors - Haihua Feng ; KD Validated
human	WB	Nucleic Acids Res; ZWC complex-mediated SPT5 phosphorylation suppresses divergent antisense RNA transcription at active gene promoters.; Authors - Kihyun Park
mouse	WB	Redox Biol; Redox-sensitive activation of CCL7 by BRG1 in hepatocytes during liver injury.; Authors - Ming Kong
human	WB	Oncogene; β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.; Authors - Yanwei Lu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Janeen (Verified Customer) (07-22-2021)

There are some background bands at high contrast. But overall, a nice clean signal at a contrast level appropriate for interpretation.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: prostate/ RWPE-1

CSNK2A1 Antibody Western Blot validation (1:5000 dilution) in prostate/ RWPE-1 (Cat no:10992-1-AP)