Anticorps Polyclonal de lapin anti-CSNK2B

CSNK2B Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 22418-1-AP

Synonymes

Casein kinase II subunit beta, CK II beta, CK2B, CK2N, CSK2B, CSNK2B, G5A, Phosvitin, Protein G5a

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Galerie de données de validation

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  WB analysis using 22418-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22418-1-AP (Casein Kinase 2 beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 22418-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22418-1-AP (Casein Kinase 2 beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 22418-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22418-1-AP (Casein Kinase 2 beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 22418-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22418-1-AP (Casein Kinase 2 beta antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 22418-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22418-1-AP (Casein Kinase 2 beta antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human thyroid using 22418-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 22418-1-AP (Casein Kinase 2 beta antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse brain using 22418-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22418-1-AP (CSNK2B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 22418-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22418-1-AP (CSNK2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid using 22418-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 22418-1-AP (Casein Kinase 2 beta antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse testis using 22418-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22418-1-AP (Casein Kinase 2 beta antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse testis using 22418-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22418-1-AP (Casein Kinase 2 beta antibody at dilution of 1:200 (under 40x lens).

• 

  FC experiment of HeLa using 22418-1-AP

  1X10^6 HeLa cells were stained with 0.2ug Casein Kinase 2 beta antibody (22418-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules K-562, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IHC: tissu testiculaire humain, tissu cérébral de souris, tissu testiculaire de souris, tissu thyroïdien humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

22418-1-AP cible CSNK2B dans les applications de WB, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CSNK2B Protéine recombinante Ag18134
• Nom complet: casein kinase 2, beta polypeptide
• Masse moléculaire calculée: 215 aa, 25 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC112017
• Symbole du gène: CSNK2B
• Identification du gène (NCBI): 1460
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CSNK2B is a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. It participates in Wnt signaling, and plays a complex role in regulating the basal catalytic activity of the alpha subunit.

Publications

Species	Application	Title
mouse	WB	Biochem Biophys Res Commun; β-catenin stimulates Tcf7l1 degradation through recruitment of casein kinase 2 in mouse embryonic stem cells.; Authors - Yan Zhang
human	WB	Cancer Metab; Phosphoproteomics revealed cellular signals immediately responding to disruption of cancer amino acid homeostasis induced by inhibition of l-type amino acid transporter 1; Authors - Hiroki Okanishi