Anticorps Polyclonal de lapin anti-CTGF

CTGF Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (4)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(49)

N° de cat : 25474-1-AP

Synonymes

CCN family member 2, CCN2, Cellular communication network factor 2, HCS24, Hypertrophic chondrocyte-specific protein 24

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Galerie de données de validation

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  WB analysis using 25474-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25474-1-AP (CTGF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HUVEC using 25474-1-AP

  IP result of anti-CTGF (IP:25474-1-AP, 4ug; Detection:25474-1-AP 1:1000) with HUVEC cells lysate 1440 ug.

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  IHC staining of mouse colon using 25474-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25474-1-AP (CTGF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 25474-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25474-1-AP (CTGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HUVEC, cellules HeLa, cellules U2OS
• Résultats positifs en IP: cellules HUVEC,
• Résultats positifs en IHC: tissu de côlon de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 45 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Informations sur le produit

25474-1-AP cible CTGF dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, canin, Chèvre, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: connective tissue growth factor
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: NM_001901
• Symbole du gène: CTGF
• Identification du gène (NCBI): 1490
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CTGF, also known as CCN2, is a member of the CCN family of matricellular proteins. CTGF, a cysteine-rich, matrix-associated, heparin-binding protein, is widely expressed in various human tissues and organs. CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers.

Publications

Species	Application	Title
human	WB	EMBO J; MST2 methylation by PRMT5 inhibits Hippo signaling and promotes pancreatic cancer progression; Authors - Yan Sun
mouse,rat	WB	Cell Death Dis; Asb10 accelerates pathological cardiac remodeling by stabilizing HSP70; Authors - Ke Lin
human	WB	Acta Pharmacol Sin; Urolithin A promotes atherosclerotic plaque stability by limiting inflammation and hypercholesteremia in Apolipoprotein E-deficient mice; Authors - Meng-Yun Xu
human	WB	Oxid Med Cell Longev; Cigarette Smoke Exposure Increases Glucose-6-phosphate Dehydrogenase, Autophagy, Fibrosis, and Senescence in Kidney Cells In Vitro and In Vivo.; Authors - Wen-Chih Liu
pig	WB	Front Bioeng Biotechnol; Effect of stem cell conditional medium-loading adhesive hydrogel on TGF-β1-induced endometrial stromal cell fibrosis; Authors - Yuan Zhu
mouse	WB	Eur J Pharmacol; Palmatine alleviates cardiac fibrosis by inhibiting fibroblast activation through the STAT3 pathway; Authors - Shaoling Lin

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