Anticorps Polyclonal de lapin anti-CTGF

CTGF Polyclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (5)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

21

N° de cat : 25474-1-AP

Synonymes

CCN family member 2, CCN2, CTGF, HCS24, IBP 8, IGF binding protein 8, IGFBP 8, IGFBP8, NOV2

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Galerie de données de validation

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  WB analysis using 25474-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25474-1-AP (CTGF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 25474-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25474-1-AP (CTGF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  FC experiment of HuH-7 using 25474-1-AP

  1X10^6 HuH-7 cells were intracellularly stained with 0.4 ug Anti-Human CTGF (25474-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HUVEC, cellules HeLa, cellules U2OS
• Résultats positifs en cytométrie: cellules HuH-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 21 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

25474-1-AP cible CTGF dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, canin, Chèvre, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: connective tissue growth factor
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: NM_001901
• Symbole du gène: CTGF
• Identification du gène (NCBI): 1490
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CTGF, also known as CCN2, is a member of the CCN family of matricellular proteins. CTGF, a cysteine-rich, matrix-associated, heparin-binding protein, is widely expressed in various human tissues and organs. CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers.

Publications

Species	Application	Title
human	WB	Oxid Med Cell Longev; Cigarette Smoke Exposure Increases Glucose-6-phosphate Dehydrogenase, Autophagy, Fibrosis, and Senescence in Kidney Cells In Vitro and In Vivo.; Authors - Wen-Chih Liu
pig	WB	Front Bioeng Biotechnol; Effect of stem cell conditional medium-loading adhesive hydrogel on TGF-β1-induced endometrial stromal cell fibrosis; Authors - Yuan Zhu
rat	WB	Chin Med; Downregulation of lncRNA Miat contributes to the protective effect of electroacupuncture against myocardial fibrosis.; Authors - Wenchuan Qi
mouse	WB,IF	J Agric Food Chem; Krill Oil Turns Off TGF-β1 Profibrotic Signaling in the Prevention of Diabetic Nephropathy; Authors - Xuechun Sun
human,mouse	WB,IHC	Front Pharmacol; 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside ameliorates bleomycin-induced pulmonary fibrosis via regulating pro-fibrotic signaling pathways; Authors - Tsung-Teng Huang
mouse	WB	Life Sci; Verteporfin inhibits the dedifferentiation of tubular epithelial cells via TGF-β1/Smad pathway but induces podocyte loss in diabetic nephropathy; Authors - Chenyang Qi