Anticorps Monoclonal anti-Beta Catenin

• Phare
• Validé par KD/KO

Beta Catenin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(121)

CloneNo.

1B8A1

Avis (1)

N° de cat : 66379-1-Ig

Synonymes

Catenin beta-1, Catenin beta 1, beta-Catenin, B-catenin, b cat

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Galerie de données de validation

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  WB analysis using 66379-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66379-1-Ig (Beta Catenin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis using 66379-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66379-1-Ig (beta-Catenin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 66379-1-Ig

  IP result of anti-Beta Catenin (IP:66379-1-Ig, 4ug; Detection:66379-1-Ig 1:2000) with mouse brain tissue lysate 4400 ug.

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  IHC staining of mouse colon using 66379-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 66379-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 66379-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 66379-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 66379-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:40000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 66379-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66379-1-Ig (beta-Catenin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66379-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66379-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 66379-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 66379-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon cancer using 66379-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Beta Catenin antibody (66379-1-Ig, Clone: 1B8A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human skin cancer using 66379-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using 66379-1-Ig (beta-Catenin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human skin cancer using 66379-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using 66379-1-Ig (beta-Catenin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of MCF-7 using 66379-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (66379-1-Ig, Clone: 1B8A1 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red).

• 

  FC experiment of MCF-7 using 66379-1-Ig

  1X10^6 MCF-7 cells were intracellularly stained with 0.5 ug Anti-Human Beta Catenin (66379-1-Ig, Clone:1B8A1) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules LNCaP, cellules MCF-7, cellules NIH/3T3, tissu cérébral de lapin, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu de côlon de souris, tissu de cancer de la peau humain, tissu de cancer du sein humain, tissu de côlon de rat, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du côlon humain, tissu de cancer de la peau humain
• Résultats positifs en IF/ICC: cellules MCF-7, tissu de cancer de la peau humain, tissu de cancer du foie humain
• Résultats positifs en FC (Intra): cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:10000-1:40000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:750-1:3000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 101 publications below
• IHC: See 12 publications below
• IF: See 36 publications below
• IP: See 1 publications below
• CoIP: See 7 publications below

Informations sur le produit

66379-1-Ig cible Beta Catenin dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, porc, poulet, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: catenin (cadherin-associated protein), beta 1, 88kDa
• Masse moléculaire calculée: 781 aa, 86 kDa
• Poids moléculaire observé: 92 kDa
• Numéro d’acquisition GenBank: NM_001904
• Symbole du gène: Beta Catenin
• Identification du gène (NCBI): 1499
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

Publications

Species	Application	Title
human	WB	Adv Mater; Noninvasive Optogenetics Realized by iPSC-Derived Tentacled Carrier in Alzheimer's Disease Treatment; Authors - Yuewen Zhai
mouse,rat	WB	Acta Pharm Sin B; Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.; Authors - Xunxun Wu
human	WB	J Exp Clin Cancer Res; Membrane RRM2-positive cells represent a malignant population with cancer stem cell features in intrahepatic cholangiocarcinoma; Authors - Yongzhi Zhao ; KD Validated
human	IF	Research (Wash D C); The Mechanics of Tumor Cells Dictate Malignancy via Cytoskeleton-Mediated APC/Wnt/β-Catenin Signaling; Authors - Xi Chen
human	IF	Acta Biomater; Microenvironmental reprogramming of human dermal papilla cells for hair follicle tissue engineering; Authors - Zhen Liu
mouse	IF	Redox Biol; Riboflavin deficiency leads to irreversible cellular changes in the RPE and disrupts retinal function through alterations in cellular metabolic homeostasis.; Authors - Tirthankar Sinha

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Bruna (Verified Customer) (05-30-2024)

Great antibody! In our lab, it was used for Western blot and immunofluorescence detection. In both cases, the results were excellent.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Cancer- associated fibroblasts.

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