Anticorps Polyclonal de lapin anti-CTP synthase

• Phare
• Validé par KD/KO

CTP synthase Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(25)

N° de cat : 15914-1-AP

Synonymes

CTPS, CTPS 1, CTPS1, EC:6.3.4.2, UTP--ammonia ligase 1

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Galerie de données de validation

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  WB analysis using 15914-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15914-1-AP (CTP synthase antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15914-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15914-1-AP (CTP synthase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15914-1-AP

  IP result of anti-CTP synthase (IP:15914-1-AP, 4ug; Detection:15914-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IHC staining of human liver cancer using 15914-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15914-1-AP (CTP synthase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15914-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15914-1-AP (CTP synthase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15914-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15914-1-AP (CTP synthase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15914-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15914-1-AP (CTP synthase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15914-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CTP synthase antibody (15914-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 15914-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CTP synthase Polyclonal antibody (15914-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules Raji
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IHC: See 2 publications below
• IF: See 12 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

15914-1-AP cible CTP synthase dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, Moustique, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CTP synthase Protéine recombinante Ag8707
• Nom complet: CTP synthase
• Masse moléculaire calculée: 591 aa, 67 kDa
• Poids moléculaire observé: 67 kDa
• Numéro d’acquisition GenBank: BC009408
• Symbole du gène: CTPS
• Identification du gène (NCBI): 1503
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CTP synthase(CTPS) is also named as CTPS1 and belongs to the CTP synthase family. It catalyses the ATP-dependent formation of CTP from UTP using either L-glutamine or NH3 as the nitrogen source(PMID:12752439). It is important in the biosynthesis of phospholipids and nucleic acids, and plays a key role in cell growth, development, and tumorigenesis (PMID:8813694). CTP synthetase exists as a dimer in the absence of its substrates ATP and UTP, but in the presence of saturating concentrations of these substrates the enzyme exists as a tetramer.(PMID:18439916)

Publications

Species	Application	Title
human	WB	Cancer Res; Combined inactivation of CTPS1 and ATR is synthetically lethal to MYC-overexpressing cancer cells.; Authors - Zhe Sun
human	IF	FEBS J; Inter-filament interaction between IMPDH and CTPS cytoophidia.; Authors - Chia-Chun Chang
human	WB,CoIP	J Cell Physiol; Energy deficiency caused by CTPS downregulation in decidua may contribute to pre-eclampsia by impairing decidualization.; Authors - Ke Ma
human		Front Immunol; Flow Cytometry Contributions for the Diagnosis and Immunopathological Characterization of Primary Immunodeficiency Diseases With Immune Dysregulation.; Authors - Otavio Cabral-Marques
human	WB	Oncotarget; Identification and validation of differentially expressed proteins in epithelial ovarian cancers using quantitative proteomics.; Authors - Hong Qu
human	IF	J Cell Sci; Rod and Ring formation from IMP dehydrogenase is regulated via the one-carbon metabolic pathway.; Authors - S John Calise

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