Anticorps Polyclonal de lapin anti-CUGBP1

• Phare
• Validé par KD/KO

CUGBP1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 13002-1-AP

Synonymes

Bruno like protein 2, BRUNOL2, CELF 1, CELF1, CUG BP, CUG BP1, CUGBP, CUGBP1, Deadenylation factor CUG BP, EDEN BP homolog, hNab50, NAB50, RNA binding protein BRUNOL 2

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Galerie de données de validation

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  WB analysis using 13002-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13002-1-AP (CUGBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13002-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13002-1-AP (CUGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 13002-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13002-1-AP (CUGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 13002-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 13002-1-AP (CUGBP1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 13002-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13002-1-AP (CUGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13002-1-AP

  IP Result of anti-CUGBP1 (IP:13002-1-AP, 4ug; Detection:13002-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human breast cancer using 13002-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13002-1-AP (CUGBP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 13002-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13002-1-AP (CUGBP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 13002-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13002-1-AP (CUGBP1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin cancer using 13002-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13002-1-AP (CUGBP1 Antibody) at dilution of 1:50.

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  IHC staining of human skin cancer using 13002-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13002-1-AP (CUGBP1 Antibody) at dilution of 1:50.

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  IHC staining of human breast cancer using 13002-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13002-1-AP (CUGBP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 13002-1-AP

  Immunofluorescent analysis of Hela cells, using CUGBP1 antibody 13002-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules HeLa, cellules HT-1080, cellules NIH/3T3, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de la peau humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

13002-1-AP cible CUGBP1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CUGBP1 Protéine recombinante Ag3677
• Nom complet: CUG triplet repeat, RNA binding protein 1
• Masse moléculaire calculée: 483 aa, 52 kDa
• Poids moléculaire observé: 51-55 kDa
• Numéro d’acquisition GenBank: BC031079
• Symbole du gène: CUGBP1
• Identification du gène (NCBI): 10658
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CUG triplet repeat RNA binding protein 1 (CUGBP1) is a member of the CUGBP Elav-like family (CELF), which plays an important role in cardiac development and function (PMID: 17130239). CUGBP1 has also been shown to localize to cytoplasmic stress granules when cells are stressed.

Publications

Species	Application	Title
mouse	IHC	Hum Mol Genet; CGG repeats in RNA modulate expression of TDP-43 in mouse and fly models of fragile X tremor ataxia syndrome.; Authors - Jocelyn N Galloway
human	WB	Carcinogenesis; RNA-binding protein CUGBP1 controls the differential INSR splicing in molecular subtypes of breast cancer cells and affects cell aggressiveness.; Authors - Gena Huang ; KD Validated
human	WB	Sci Rep; DLX6-AS1 activated by H3K4me1 enhanced secondary cisplatin resistance of lung squamous cell carcinoma through modulating miR-181a-5p/miR-382-5p/CELF1 axis.; Authors - Xu Zhao ; KD Validated
human,mouse	WB	Bioengineered; Guizhi Fuling pill attenuates liver fibrosis in vitro and in vivo via inhibiting TGF-β1/Smad2/3 and activating IFN-γ/Smad7 signaling pathways.; Authors - Zhongliang Liu