Anticorps Polyclonal de lapin anti-CUL1

• Phare
• Validé par KD/KO

CUL1 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(24)

Avis (1)

N° de cat : 12895-1-AP

Synonymes

CUL-1, Cullin1, Cullin-1

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Galerie de données de validation

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  WB analysis using 12895-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12895-1-AP (CUL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 12895-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12895-1-AP (CUL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 12895-1-AP

  IP result of anti-CUL1 (IP:12895-1-AP, 4ug; Detection:12895-1-AP 1:2000) with K-562 cells lysate 1760 ug.

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  IP experiment of HeLa using 12895-1-AP

  IP result of anti-CUL1 (IP:12895-1-AP, 3ug; Detection:12895-1-AP 1:300) with HeLa cells lysate 2000ug.

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  IHC staining of human lung cancer using 12895-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12895-1-AP (CUL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12895-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12895-1-AP (CUL1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HeLa using 12895-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CUL1 (12895-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules BxPC-3, cellules K-562
• Résultats positifs en IP: cellules K-562, cellules HeLa
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 24 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

12895-1-AP cible CUL1 dans les applications de WB, IHC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CUL1 Protéine recombinante Ag3585
• Nom complet: cullin 1
• Masse moléculaire calculée: 90 kDa
• Poids moléculaire observé: 80-90 kDa
• Numéro d’acquisition GenBank: BC034318
• Symbole du gène: CUL1
• Identification du gène (NCBI): 8454
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.

Publications

Species	Application	Title
human	WB	Nat Chem Biol; E2-Ub-R74G strategy reveals E2-specific ubiquitin conjugation profiles in live cells; Authors - Siqi Shen
mouse	WB	Redox Biol; Cardioprotective effect of MLN4924 on ameliorating autophagic flux impairment in myocardial ischemia-reperfusion injury by Sirt1.; Authors - Ji Zhang
human	WB	Front Bioeng Biotechnol; Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts.; Authors - Kaile Zhang
mouse	WB,IP	Pharmacol Res; Cullin-associated and neddylation-dissociated 1 protein (CAND1) governs cardiac hypertrophy and heart failure partially through regulating calcineurin degradation.; Authors - Xingda Li
human	WB	iScience; A small-molecule Skp1 inhibitor elicits cell death by p53-dependent mechanism.; Authors - Muzammal Hussain
human	WB	J Cell Sci; Cullin-3-KCTD10-mediated CEP97 degradation promotes primary cilium formation.; Authors - Tomoaki Nagai ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

WEI (Verified Customer) (05-11-2022)

strong band for predicted band

• Applications: Western Blot
• Cell Tissue Type: Mouse Heart

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