Anticorps Monoclonal anti-CUL2

• Phare
• Validé par KD/KO

CUL2 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

1A6G9

N° de cat : 67175-1-Ig

Synonymes

CUL 2, CUL2, cullin 2

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Galerie de données de validation

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  WB analysis of MCF-7 using 67175-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 67175-1-Ig

  WB result of CUL2 antibody (67175-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL2 transfected Raji cells.

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  WB analysis of PC-3 using 67175-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67175-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67175-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67175-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67175-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67175-1-Ig (CUL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67175-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67175-1-Ig (CUL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 67175-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67175-1-Ig (CUL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 67175-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67175-1-Ig (CUL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 67175-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CUL2 antibody (67175-1-Ig, Clone: 1A6G9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules PC-3, cellules Raji
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67175-1-Ig cible CUL2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CUL2 Protéine recombinante Ag28654
• Nom complet: cullin 2
• Masse moléculaire calculée: 87 kDa
• Poids moléculaire observé: 85-90 kDa
• Numéro d’acquisition GenBank: BC009591
• Symbole du gène: CUL2
• Identification du gène (NCBI): 8453
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proteins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.