Anticorps Polyclonal de lapin anti-CUL4B

• Phare
• Validé par KD/KO

CUL4B Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(84)

N° de cat : 12916-1-AP

Synonymes

KIAA0695, Cullin-4B, cullin 4B

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Galerie de données de validation

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  WB analysis using 12916-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12916-1-AP (CUL4B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa cells using 12916-1-AP

  WB result of CUL4B antibody (12916-1-AP, 1:2,000) with si-Control and si-CUL4B transfected HeLa cells.

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  IP experiment of HeLa using 12916-1-AP

  IP result of anti-CUL4B (IP:12916-1-AP, 3ug; Detection:12916-1-AP 1:1000) with HeLa cells lysate 4650ug.

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  IP experiment of HeLa using 12916-1-AP

  IP result of anti-CUL4B (IP:12916-1-AP, 4ug; Detection:12916-1-AP 1:5000) with HeLa cells lysate 1280 ug.

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  IF Staining of HeLa using 12916-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUL4B antibody (12916-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 12916-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUL4B antibody (12916-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 12916-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUL4B antibody (12916-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 29 publications below
• WB: See 79 publications below
• IHC: See 4 publications below
• IF: See 8 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

12916-1-AP cible CUL4B dans les applications de WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CUL4B Protéine recombinante Ag3563
• Nom complet: cullin 4B
• Masse moléculaire calculée: 913 aa, 104 kDa
• Poids moléculaire observé: 102 kDa
• Numéro d’acquisition GenBank: BC036216
• Symbole du gène: CUL4B
• Identification du gène (NCBI): 8450
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329). This antibody may detect both CUL4A (87 kDa) and CUL4B(104 kDa).

Publications

Species	Application	Title
human	WB	Nature; Targeted protein degradation via intramolecular bivalent glues; Authors - Oliver Hsia ; KD Validated
human	CoIP,WB	Cell; FBXO44 promotes DNA replication-coupled repetitive element silencing in cancer cells.; Authors - Jia Z Shen ; KD Validated
human	WB	Cell; Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.; Authors - Xiulei Mo
human	IHC,IF	Science; CRL4 complex regulates mammalian oocyte survival and reprogramming by activation of TET proteins.; Authors - Chao Yu
human	WB	Nat Med; Immunomodulatory drugs disrupt the cereblon-CD147-MCT1 axis to exert antitumor activity and teratogenicity.; Authors - Ruth Eichner ; KD Validated
human	WB	Science; Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15.; Authors - Ting Han

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