Anticorps Polyclonal de lapin anti-CWC15

CWC15 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 25293-1-AP

Synonymes

Protein CWC15 homolog, ORF5, HSPC148, Cwf15, C11orf5

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Galerie de données de validation

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  WB analysis of mouse liver using 25293-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 25293-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 25293-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25293-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 25293-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 25293-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 25293-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SW 1990 using 25293-1-AP

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 25293-1-AP

  IP result of anti-CWC15 (IP:25293-1-AP, 3ug; Detection:25293-1-AP 1:600) with HeLa cells lysate 2800ug.

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  IHC staining of human stomach using 25293-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 25293-1-AP (CWC15 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 25293-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25293-1-AP (CWC15 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 25293-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25293-1-AP (CWC15 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 25293-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 25293-1-AP (CWC15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HeLa, cellules HepG2, cellules Jurkat, cellules PC-3, cellules SW-1990, tissu cardiaque de souris, tissu testiculaire de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu d'estomac humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

25293-1-AP cible CWC15 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CWC15 Protéine recombinante Ag18116
• Nom complet: CWC15 spliceosome-associated protein homolog (S. cerevisiae)
• Masse moléculaire calculée: 229 aa, 27 kDa
• Poids moléculaire observé: 27-35 kDa
• Numéro d’acquisition GenBank: BC040946
• Symbole du gène: CWC15
• Identification du gène (NCBI): 51503
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CWC15, also named as C11orf5 or Spliceosome-associated protein CWC15 homolog, is a 229 amino acid protein, which belongs to the CWC15 family. CWC15 as a Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing.

Publications

Species	Application	Title
	WB	Nucleic Acids Res; CTNNBL1 facilitates the association of CWC15 with CDC5L and is required to maintain the abundance of the Prp19 spliceosomal complex.; Authors - Febe van Maldegem

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