Anticorps Polyclonal de lapin anti-CX3CL1

CX3CL1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(17)

N° de cat : 10108-2-AP

Synonymes

C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, C3Xkine, C X3 C motif chemokine 1, ABCD 3

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Galerie de données de validation

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  WB analysis of PC-3 using 10108-2-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10108-2-AP (CX3CL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10108-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10108-2-AP (CX3CL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 10108-2-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10108-2-AP (CX3CL1 antibody) at dilution of 1:400 (under 10x lens)..

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  IHC staining of human small intestine using 10108-2-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10108-2-AP (CX3CL1 antibody) at dilution of 1:400 (under 40x lens)..

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  IHC staining of human lung using 10108-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 10108-2-AP (CX3CL1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 10108-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 10108-2-AP (CX3CL1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules PC-3, tissu cérébral humain
• Résultats positifs en IHC: tissu d'intestin grêle humain, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 9 publications below

Informations sur le produit

10108-2-AP cible CX3CL1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CX3CL1 Protéine recombinante Ag0161
• Nom complet: chemokine (C-X3-C motif) ligand 1
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: BC001163
• Symbole du gène: CX3CL1
• Identification du gène (NCBI): 6376
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Chemokines direct the trafficking of white blood cells in immune surveillance, playing a key role in inflammatory and infectious diseases such as AIDS. The human CX3C chemokine carries the chemokine domain on top of an extended mucin-like stalk. CX3CL1 is synthesized as a 50-75 kDa precursor and undergoes glycosylation to yield two mature forms: a 100 kDa cell membrane bound form and an 85 kDa soluble form which is released from the cell surface. The soluble CX3C chemokine has potent chemoattractant activity for T cells and monocytes, and the cell-surface-bound protein, which is induced by primary proinflammatory signals in activated primary endothelial cells, promotes strong adhesion of those leukocytes.

Publications

Species	Application	Title
human	IHC	Mol Cancer; YTHDF2 in peritumoral hepatocytes mediates chemotherapy-induced antitumor immune responses through CX3CL1-mediated CD8+ T cell recruitment; Authors - Zhenyun Yang
human	IHC	Blood; Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes.; Authors - Ma Yue Y
human	IHC	J Pathol; Current smoking-specific gene expression signature in normal bronchial epithelium is enhanced in squamous cell lung cancer.; Authors - Boelens Mirjam C MC
human	WB	Mol Oncol; Platelets are recruited to hepatocellular carcinoma tissues in a CX3CL1-CX3CR1 dependent manner and induce tumour cell apoptosis.; Authors - Shuo Miao
human	WB	Int J Biol Sci; Long noncoding RNA SH3PXD2A-AS1 promotes colorectal cancer progression by regulating p53-mediated gene transcription.; Authors - Pingfu Hou
Human	IHC	Eur J Med Res; HCC-derived CX3CL1 affects hepatocellular carcinoma prognosis and CX3CR1 + MDSC infiltration; Authors - Xiaoling Zhang

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