Anticorps Polyclonal de lapin anti-CX3CR1

• Phare
• Validé par KD/KO

CX3CR1 Polyclonal Antibody for FC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA

Conjugaison

Non conjugué

Publications

30

Avis (2)

N° de cat : 13885-1-AP

Synonymes

Beta chemokine receptor like 1, C X3 C CKR 1, CCRL1, CMK BRL 1, CMK BRL1, CMKBRL1, CMKDR1, CX3C chemokine receptor 1, CX3CR1, Fractalkine receptor, G protein coupled receptor 13, GPR13, GPRV28, V28

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Galerie de données de validation

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  WB analysis of HEK-293 using 13885-1-AP

  WB result of CX3CR1 antibody (13885-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CX3CR1 transfected HEK-293 cells.

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  WB analysis using 13885-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13885-1-AP (CX3CR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 13885-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13885-1-AP (CX3CR1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 13885-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13885-1-AP (CX3CR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 13885-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 13885-1-AP (CX3CR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13885-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13885-1-AP (CX3CR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 13885-1-AP

  IP Result of anti-CX3CR1 (IP:13885-1-AP, 4ug; Detection:13885-1-AP 1:1000) with SH-SY5Y cells lysate 1600ug.

• 

  FC experiment of K-562 using 13885-1-AP

  1X10^6 K-562 cells were stained with 0.2ug CX3CR1 antibody (13885-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HL-60, cellules K-562, cellules SH-SY5Y, cellules U-937
• Résultats positifs en IP: cellules SH-SY5Y
• Résultats positifs en cytométrie: cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 22 publications below
• IHC: See 11 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

13885-1-AP cible CX3CR1 dans les applications de WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CX3CR1 Protéine recombinante Ag4149
• Nom complet: chemokine (C-X3-C motif) receptor 1
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 44-47 kDa
• Numéro d’acquisition GenBank: BC028078
• Symbole du gène: CX3CR1
• Identification du gène (NCBI): 1524
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CX3CR1, also known as GPR13, V28 and fractalkine receptor, belongs to the 7-transmembrane G protein-coupled receptor (GPCR) family. It is expressed on microglia, astrocytes, NK cells, monocytes/macrophages, and a subpopulation of T cells. CX3CR1 is the receptor for fractalkine (CX3CL1) and mediates both its adhesive and migratory functions. It also acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro), and some variations in the gene of CX3CR1 lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Defects in CX3CR1 are a cause of susceptibility to age-related macular degeneration type 12 (ARMD12).

Publications

Species	Application	Title
mouse	IP	Brain; Reactive microglia enhance the transmission of exosomal alpha-synuclein via toll-like receptor 2.; Authors - Yun Xia
human	WB,IF	Tissue Cell; Analysis of Aβ-induced neurotoxicity and microglial responses in simple two- and three-dimensional human iPSC-derived cortical culture systems; Authors - Mayuko Takata
mouse	WB	J Control Release; Chemoattractants driven and microglia based biomimetic nanoparticle treating TMZ-resistant glioblastoma multiforme.; Authors - Sai Qiao
mouse	WB	Nanoscale; Calming egress of inflammatory monocytes and related septic shock by therapeutic CCR2 silencing using macrophage-derived extracellular vesicles.; Authors - Liang Ding
human	WB	Mol Oncol; Platelets are recruited to hepatocellular carcinoma tissues in a CX3CL1-CX3CR1 dependent manner and induce tumour cell apoptosis.; Authors - Shuo Miao
mouse	WB,Cell treatment	J Nanobiotechnology; Biomimetic GBM-targeted drug delivery system boosting ferroptosis for immunotherapy of orthotopic drug-resistant GBM.; Authors - Bao Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Reyes (Verified Customer) (03-01-2024)

Cx3cr1 antibody (in red) tried on human paraffin brain sections, apparently vesicular while marking my microglia.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

CX3CR1 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:13885-1-AP)

FH

Ryan (Verified Customer) (06-15-2018)

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: zebrafish retina

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