Anticorps Polyclonal de lapin anti-CXCL13/BCA1

CXCL13/BCA1 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(26)

N° de cat : 10927-1-AP

Synonymes

CXCL13, chemokine (C X C motif) ligand 13, C X C motif chemokine 13, BCA-1, BCA1

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Galerie de données de validation

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  IHC staining of human tonsillitis using 10927-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10927-1-AP (CXCL13/BCA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 10927-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10927-1-AP (CXCL13/BCA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 10927-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10927-1-AP (CXCL13/BCA1 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 10927-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10927-1-AP (CXCL13/BCA1 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lymphoma using 10927-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10927-1-AP (CXCL13/BCA1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 10927-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10927-1-AP (CXCL13/BCA1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10927-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10927-1-AP (CXCL13/BCA1 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 10927-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10927-1-AP (CXCL13/BCA1 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 10927-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10927-1-AP (CXCL13/BCA1 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 10927-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10927-1-AP (CXCL13/BCA1 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 10927-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10927-1-AP (CXCL13/BCA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 10927-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10927-1-AP (CXCL13/BCA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of TT using 10927-1-AP

  1x10^6 TT cells were intracellularly stained with 0.2 ug CXCL13/BCA1 Polyclonal antibody (10927-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer du sein humain, tissu de carcinome à cellules rénales humain, tissu de lymphome humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): TT cells,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 15 publications below
• IF: See 6 publications below

Informations sur le produit

10927-1-AP cible CXCL13/BCA1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CXCL13/BCA1 Protéine recombinante Ag1358
• Nom complet: chemokine (C-X-C motif) ligand 13
• Masse moléculaire calculée: 13 kDa
• Numéro d’acquisition GenBank: BC012589
• Symbole du gène: CXCL13/BCA1
• Identification du gène (NCBI): 10563
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BLR1 (Burkitt lymphoma receptor-1), also called CXCR5, is highly expressed on Burkitt lymphoma cells and B lymphocytes. It may therefore function in the homing of B lymphocytes to follicles. It was reported that the B cell chemokine CXCL13 is elevated in MS serum and cerebrospinal fluid. CXCL13 is also regarded as a proposed serum biomarker of synovitis in RA( Rheumatoid arthritis). CXCL13 consists of a 22-aa signal peptide and a 87-aa mature poly-peptide.

Publications

Species	Application	Title
human	IHC	Sci Adv; Defined lifestyle and germline factors predispose Asian populations to gastric cancer.; Authors - Akihiro Suzuki
human	IF	Nat Commun; B cells sustain inflammation and predict response to immune checkpoint blockade in human melanoma.; Authors - Johannes Griss
human	IHC	Genomics; MIN score predicts primary response to infliximab/adalimumab and vedolizumab therapy in patients with inflammatory bowel diseases.; Authors - Yuan Shi
human	WB	Aging (Albany NY); Immune profiling reveals prognostic genes in high-grade serous ovarian cancer.; Authors - Yong Wu
human	IHC,IF	J Immunol; Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease.; Authors - Kim Mi Jeong MJ
human	IHC，IF	Front Immunol; A Standardized Analysis of Tertiary Lymphoid Structures in Human Melanoma: Disease Progression- and Tumor Site-Associated Changes With Germinal Center Alteration.; Authors - Franziska Werner

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