Anticorps Recombinant de lapin anti-CXCL9/MIG

CXCL9/MIG Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

APC-Cyanine7 Fluorescent Dye

CloneNo.

241437F5

N° de cat : AY7-FcA98211

Synonymes

CXCL9, MIG, MIG; CXCL9, 241437F5, CMK

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Galerie de données de validation

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  FC experiment of human PBMCs using AY7-FcA98211

  1x10^6 untreated or IFN gamma, TNF α and Brefeldin A treated human PBMCs were intracellularly stained with FcZero-rAb™ CoraLite® Plus 488 Anti-Human CD14 and 5 ul APC-Cyanine7 Anti-Human CXCL9/MIG Rabbit RecAb (AY7-FcA98211, Clone:241437F5). Cells were incubated with MonoZero™ Monocytes blocking Reagent(PF00020) prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of human PBMCs using AY7-FcA98211

  1x10^6 IFN gamma, TNF α and Brefeldin A treated human PBMCs were intracellularly stained with 5 ul APC-Cyanine7 Anti-Human CXCL9/MIG Rabbit RecAb (AY7-FcA98211, Clone: 241437F5) (red) or FcZero-rAb™ APC-Cyanine7 Rabbit IgG Isotype Control Recombinant Antibody (AY7-FcA98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were intracellularly stained with 5 ul APC-Cyanine7 Anti-Human CXCL9/MIG Rabbit RecAb (AY7-FcA98211, Clone: 241437F5) (black, dashed). Cells were incubated with MonoZero™ Monocytes blocking Reagent(PF00020) prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en FC (Intra): IFN gamma, TNF α and Brefeldin A treated human PBMCs,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

AY7-FcA98211 cible CXCL9/MIG dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CXCL9/MIG Protéine recombinante Ag17932
• Nom complet: chemokine (C-X-C motif) ligand 9
• Masse moléculaire calculée: 14 kDa
• Numéro d’acquisition GenBank: BC095396
• Symbole du gène: CXCL9
• Identification du gène (NCBI): 4283
• Conjugaison: APC-Cyanine7 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 778 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CXCL9 is a small cytokine known as MIG. CXCL9 is a T-cell chemoattractant, which is induced by IFN-γ. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4. A high level of CXCL9 revealed in peripheral liquids, can be considered as a marker of host immune response, especially of that involving Th1 cells.

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