Anticorps Polyclonal de lapin anti-CXCR3

CXCR3 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 26756-1-AP

Synonymes

CD183, CKR L2, CMKAR3, CXC R3, CXCR 3, CXCR3, CXCR3A, CXCR3B, G protein coupled receptor 9, GPR9, IP 10 receptor, IP10 R, Mig R, MigR

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Galerie de données de validation

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  WB analysis of HepG2 using 26756-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26756-1-AP (CXCR3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 26756-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26756-1-AP (CXCR3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 26756-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 26756-1-AP (CXCR3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 26756-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26756-1-AP (CXCR3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 26756-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26756-1-AP (CXCR3 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 26756-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26756-1-AP (CXCR3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 26756-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26756-1-AP (CXCR3 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules Jurkat, tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 5 publications below

Informations sur le produit

26756-1-AP cible CXCR3 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CXCR3 Protéine recombinante Ag25056
• Nom complet: chemokine (C-X-C motif) receptor 3
• Masse moléculaire calculée: 368 aa, 41 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC034403
• Symbole du gène: CXCR3
• Identification du gène (NCBI): 2833
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CXCR3 (C-X-C chemokine receptor type 3) is a G-protein-coupled seven-transmembrane domain chemokine receptor that is expressed on the surface of a number of cell types, including activated CD4+ and CD8+ T cells, NK and NK-T cells, plasmacytoid dendritic cells, and some B cells. CXCR3 is activated by three related chemokines (CXCL9, CXCL10, and CXCL11) and plays an important role in effector T-cell and NK cell trafficking. CXCR3 has three isoforms termed as CXCR3-A, CXCR3-B and CXCR3-alt with the calculated molecular mass of 41kDa, 46kDa and 29kDa respectively. The apparent molecular mass of CXCR3 detected by some scientists is 70kDa, but 45kDa by some others. (PMID: 16847335, 15150261, 22885102, 19151743)

Publications

Species	Application	Title
human	WB	Bioeng Transl Med; Therapeutic potential of nanoceria pretreatment in preventing the development of urological chronic pelvic pain syndrome: Immunomodulation via reactive oxygen species scavenging and SerpinB2 downregulation; Authors - Wei-Chih Lien
human	WB	J Cell Sci; Gap junction-mediated MiR-200b on osteogenesis and angiogenesis in coculture between MSCs and HUVECs.; Authors - Xiaoting Fan
human	WB	J Immunol; A Novel TrxR1 Inhibitor Regulates NK and CD8+ T Cell Infiltration and Cytotoxicity, Enhancing the Efficacy of Anti-PD-1 Immunotherapy against Hepatocarcinoma; Authors - Xiaoxia Su
mouse	WB,IF	Front Pharmacol; Paeonol Ameliorates Chronic Itch and Spinal Astrocytic Activation via CXCR3 in an Experimental Dry Skin Model in Mice.; Authors - Wen Wang
human,mouse	IF	Front Endocrinol (Lausanne); Induction of Collagen I by CXCL10 in Ovarian Theca-Stroma Cells via the JNK Pathway.; Authors - Chaojun Wang
human	WB	Autoimmunity; CXCL4 promoted the production of CD4+CD25+FOXP3+treg cells in mouse sepsis model through regulating STAT5/FOXP3 pathway.; Authors - Tao Xu