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Anticorps Polyclonal de lapin anti-CXCR7-Specific

CXCR7-Specific Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC,ELISA

Conjugaison

Non conjugué

N° de cat : 14840-1-AP

Synonymes

Galerie de données de validation

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  • Western Blot (WB) analysis of K-562 cells using CXCR7-Specific Polyclonal antibody (14840-1-AP)

    WB analysis of K-562 using 14840-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14840-1-AP (CXCR7-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C6 cells using CXCR7-Specific Polyclonal antibody (14840-1-AP)

    WB analysis of C6 using 14840-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 14840-1-AP (CXCR7-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CXCR7-Specific Polyclonal antibody (14840-1-AP)

    WB analysis of mouse brain using 14840-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14840-1-AP (CXCR7-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using CXCR7-Specific Polyclonal antibody (14840-1-AP)

    IP experiment of K-562 using 14840-1-AP

    IP Result of anti-CXCR7-Specific (IP:14840-1-AP, 4ug; Detection:14840-1-AP 1:500) with K-562 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CXCR7-Specific Polyclonal antibody (14840-1-AP)

    IHC staining of mouse brain using 14840-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14840-1-AP (CXCR7-Specific Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CXCR7-Specific Polyclonal antibody (14840-1-AP)

    IHC staining of mouse brain using 14840-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14840-1-AP (CXCR7-Specific Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Applications testées

Résultats positifs en WBcellules K-562, cellules C6, tissu cérébral de souris
Résultats positifs en IPcellules K-562
Résultats positifs en IHCtissu cérébral de souris
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

14840-1-AP cible CXCR7-Specific dans les applications de WB, IP, IHC,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Peptide
Nom complet chemokine (C-X-C motif) receptor 7
Masse moléculaire calculée 41 kDa
Poids moléculaire observé 50 kDa
Numéro d’acquisition GenBankNM_020311
Symbole du gène CXCR7
Identification du gène (NCBI) 57007
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CXCR7 (C-X-C chemokine receptor type 7), also known as RDC1, is a member of the G-protein coupled receptor family. CXCR7 can bind the chemokines CXCL11 and CXCL12 with high affinity, and it also acts as coreceptor with CXCR4 for a restricted number of HIV isolates. Expression of CXCR7 has been associated with cardiac development as well as with tumor progression. This antibody is specific to CXCR7.

Protocole

Product Specific Protocols
WB protocol for CXCR7-Specific antibody 14840-1-APDownload protocol
IHC protocol for CXCR7-Specific antibody 14840-1-APDownload protocol
IP protocol for CXCR7-Specific antibody 14840-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols