Anticorps Polyclonal de lapin anti-CYC1

• Phare
• Validé par KD/KO

CYC1 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (3)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

30

Avis (1)

N° de cat : 10242-1-AP

Synonymes

Complex III subunit 4, Complex III subunit IV, CYC1, cytochrome c 1, UQCR4

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Galerie de données de validation

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  WB analysis of human brain using 10242-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10242-1-AP (CYC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10242-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10242-1-AP (CYC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 10242-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10242-1-AP (CYC1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 10242-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10242-1-AP (CYC1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 10242-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10242-1-AP (CYC1 antibody) at dilution of 1:50 (under 25x lens).

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  IHC staining of human breast cancer using 10242-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10242-1-AP (CYC1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 10242-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10242-1-AP (CYC1 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 29 publications below
• IHC: See 2 publications below

Informations sur le produit

10242-1-AP cible CYC1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, porc, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CYC1 Protéine recombinante Ag0291
• Nom complet: cytochrome c-1
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC001006
• Symbole du gène: CYC1
• Identification du gène (NCBI): 1537
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CYC1, also named as Cytochrome c-1, belongs to the cytochrome c family. Cytochrome c1 is one respiratory subunit of the 11 subunits of the cytochrome bc1 complex of the mitochondrial electron-transfer chain. It mediates the transfer of an electron from Rieske iron-sulfur protein to cytochrome c(PMID:3036122 ). CYC1 mediates apoptosis. In this pathway, a variety of apoptotic stimuli cause CYC1 release from mitochondria, which in turn induces a series of biochemical reactions that result incaspase activation and subsequent cell death.(PMID:15189137) While CYC1 release and DNA fragmentation are unaffected by the noncleavablep75 mutant, mitochondrial morphology of dying cells is maintained, and loss of plasma membrane integrity is delayed(PMID:15186778). The protein migrated as a 35 kd band in SDS page.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis.; Authors - Shotaro Saita
human	WB	EMBO J; Respiratory supercomplexes act as a platform for complex III-mediated maturation of human mitochondrial complexes I and IV.; Authors - Margherita Protasoni
human,mouse	WB	Cell Res; Caspase cleavage of cytochrome c1 disrupts mitochondrial function and enhances cytochrome c release.; Authors - Zhu Yushan Y
human	WB	Elife; Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.; Authors - Maxence Le Vasseur
mouse	WB	Redox Biol; Examination of bioenergetic function in the inner mitochondrial membrane peptidase 2-like (Immp2l) mutant mice.; Authors - Manish S Bharadwaj
human	WB	Environ Pollut; Mitochondrial respiratory chain dysfunction mediated by ROS is a primary point of fluoride-induced damage in Hepa1-6 cells.; Authors - Hong-Wei Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Daniel (Verified Customer) (09-16-2019)

The nitrocellulose membrane was blocked with PBS, 5% BSA, w/ Tween-20, yet high levels of background was observed.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse kidney, normoxic (lane1), hypoxic (lane2)

CYC1 Antibody Western Blot, validation (1:1000 dilution) in Mouse kidney, normoxic (lane1), hypoxic (lane2) (Cat no:10242-1-AP)