Anticorps Polyclonal de lapin anti-Cytochrome c

• Phare
• Validé par KD/KO

Cytochrome c Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (6)

Applications

WB, IHC, IF-P, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(628)

Avis (5)

N° de cat : 10993-1-AP

Synonymes

CYC, CYCS

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Galerie de données de validation

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  WB analysis using 10993-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10993-1-AP (Cytochrome c antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10993-1-AP

  WB result of Cytochrome c antibody (10993-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytochrome c transfected HepG2 cells.

• 

  WB analysis of HEK-293 using 10993-1-AP

  WB result of Cytochrome c antibody (10993-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytochrome c transfected HEK-293 cells.

• 

  WB analysis using 10993-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10993-1-AP (Cytochrome c antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 10993-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10993-1-AP (Cytochrome c Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10993-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10993-1-AP (CYCS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 10993-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10993-1-AP (CYCS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10993-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10993-1-AP (CYCS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 10993-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10993-1-AP (Cytochrome c antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10993-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10993-1-AP (Cytochrome c antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10993-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10993-1-AP (Cytochrome c antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10993-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10993-1-AP (Cytochrome c antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10993-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10993-1-AP (Cytochrome c antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10993-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10993-1-AP (Cytochrome c antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10993-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10993-1-AP (CYCS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10993-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10993-1-AP (CYCS antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse eye using 10993-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Cytochrome c antibody (10993-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse eye using 10993-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Cytochrome c antibody (10993-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HepG2 using 10993-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Cytochrome c (10993-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C2C12, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules NIH/3T3, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris, tissu hépatique de rat, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu cérébral de souris, tissu de cancer du côlon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu oculaire de souris,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 582 publications below
• IHC: See 33 publications below
• IF: See 38 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

10993-1-AP cible Cytochrome c dans les applications de WB, IHC, IF-P, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, porc, poulet, singe, souris, Hamster, Hippospongia
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cytochrome c Protéine recombinante Ag1455
• Nom complet: cytochrome c, somatic
• Masse moléculaire calculée: 12 kDa
• Poids moléculaire observé: 12-15 kDa
• Numéro d’acquisition GenBank: BC009578
• Symbole du gène: Cytochrome c
• Identification du gène (NCBI): 54205
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain, cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides, cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation, cytochrome c ca99n be released from mitochondria into cytoplasm, which is required for caspase-3 activation and the occurrence of apoptosis.

Publications

Species	Application	Title
human	WB	Cell; Tau interactome maps synaptic and mitochondrial processes associated with neurodegeneration.; Authors - Tara E Tracy
human	WB,IF	Nat Commun; MYG1 drives glycolysis and colorectal cancer development through nuclear-mitochondrial collaboration; Authors - Jianxiong Chen
human	WB	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang
human	WB	Adv Sci (Weinh); Mitochondrial tRNAGlu 14693A>G Mutation, an "Enhancer" to the Phenotypic Expression of Leber's Hereditary Optic Neuropathy; Authors - Lihao Jin
human,rat	WB	Adv Sci (Weinh); Hierarchical Targeting Nanodrug with Holistic DNA Protection for Effective Treatment of Acute Kidney Injury; Authors - Qiaohui Chen
mouse	WB	Acta Pharm Sin B; Honokiol alleviated neurodegeneration by reducing oxidative stress and improving mitochondrial function in mutant SOD1 cellular and mouse models of amyotrophic lateral sclerosis; Authors - Yujun Zhou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kazu (Verified Customer) (12-14-2022)

Worked well for 4% PFA fixed mouse optic nerve using this antibody at dilution of 1:200. Little background observed.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse optic nerve

FH

Azita (Verified Customer) (06-02-2021)

Western blot analysis using Cytochrome c polyclonal antibody in NSC34 cell line at dilution of 1:1000.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: NSC34

FH

Ying (Verified Customer) (04-22-2021)

good antibody. work well with mouse cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000

Cytochrome c Antibody Western Blot validation (1:4000 dilution) in (Cat no:10993-1-AP)

FH

Chi (Verified Customer) (09-26-2019)

The antibody works very well with good signal and low background.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse embryonic fibroblasts

Cytochrome c Antibody Western Blot, validation (1:2000 dilution) in mouse embryonic fibroblasts (Cat no:10993-1-AP)

FH

Xiaoping (Verified Customer) (10-22-2018)

The signal is good and the size is between 10-15kDa.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse fibroblast

Cytochrome c Antibody Western Blot, validation (1:1000 dilution) in mouse fibroblast (Cat no:10993-1-AP)

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